BLK antibody LS-C80808 is an unconjugated rabbit polyclonal antibody to BLK from human, mouse, rat and other species. Validated for WB.
Human, Monkey, Mouse, Rat, Hamster, Pig
(tested or 100% immunogen sequence identity)
Also available conjugated with Biotin
BLK antibody was raised against synthetic peptide located between aa301-350 of human BLK (P51451, NP_001706). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Pig (100%); Dog, Bovine, Rabbit, Horse, Guinea pig (92%); Galago, Platypus (85%); Chicken (84%).
ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 2.5 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
PBS, 0.09% sodium azide, 2% sucrose
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.