BLK antibody LS-B9229 is an unconjugated goat polyclonal antibody to BLK from human and monkey. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
(tested or 100% immunogen sequence identity)
- IHC - Paraffin (3.75 µg/ml)
- Western blot (0.1 - 0.3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
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BLK antibody was raised against synthetic peptide QWSPLKVSAQDKD-C from the N-terminus (near) of human BLK (NP_001706.2). Percent identity by BLAST analysis: Human, Monkey (100%); Marmoset (85%).
- Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B9229. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 50kD band observed in Human Thymus, Spleen and Lymph Node lysates (calculated MW of 57.7kD according to NP_001706.2). Recommended concentration: 0.1-0.3 ug/ml.
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.