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Antibodies > BID > LS-C413091
 
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Catalog Number Size Price
LS-C413091-100 100 µl (1 mg/ml) $321 
BID Antibody - Immunohistochemical analysis of BID staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
BID Antibody - Immunofluorescent analysis of BID staining in RAW264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
BID Antibody - Western blot analysis of BID expression in Jurkat (A); rat muscle (B) whole cell lysates.
BID Antibody - Immunohistochemical analysis of BID staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
BID Antibody - Immunofluorescent analysis of BID staining in RAW264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
BID Antibody - Western blot analysis of BID expression in Jurkat (A); rat muscle (B) whole cell lysates.
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Polyclonal Rabbit anti‑Human BID Antibody (Internal, IHC, IF, WB) LS‑C413091

Polyclonal Rabbit anti‑Human BID Antibody (Internal, IHC, IF, WB) LS‑C413091

Submit Review LSBio Guarantee
Antibody:
BID Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$321
LS-C413091-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
BID Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
BID antibody LS-C413091 is an unconjugated rabbit polyclonal antibody to BID (Internal) from human. It is reactive with human and rat. Validated for ICC, IF, IHC and WB.
Target
Human BID
Synonyms
BID | Apoptic death agonist | BID isoform L(2) | BID isoform Si6 | Bh3 | FP497 | p22 BID | BID isoform ES(1b) | Desmocollin type 4 | Human BID coding sequence
Host
Rabbit
Reactivity
Human, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the central region of human BID.
Epitope
Internal
Specificity
Recognizes endogenous levels of BID protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BID
P55957 NM_001196 NP_001187.1

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Species: Mouse, Human, Rat, Dog, Gerbil
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IHC‑plus™ BID Antibody (aa230‑241) LS‑B11168
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
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Range: 1-25 ng/ml
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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/16/2024
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