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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BHMT Antibody (aa391-402) IHC-plus™ LS-B3257

Note: This antibody replaces LS-C61713, LS-C121122
Anti-BHMT antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-BHMT antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
BHMT antibody (0.03µg/ml) staining of Rat Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Antibody (0.03 ug/ml) staining of Rat Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Anti-BHMT antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-BHMT antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
BHMT antibody (0.03µg/ml) staining of Rat Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Antibody (0.03 ug/ml) staining of Rat Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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BHMT Antibody (aa391-402) IHC-plus™ LS-B3257

Note: This antibody replaces LS-C61713, LS-C121122
Antibody:
Goat Polyclonal to Human BHMT
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Rat, Hamster
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B3257-50
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Human BHMT
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Rat, Hamster
Format:
Unconjugated, Unmodified

Specifications

Description
BHMT antibody LS-B3257 is an unconjugated goat polyclonal antibody to BHMT from human, rat, hamster and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.

Target
Human BHMT
Host
Goat
Reactivity
Human, Monkey, Rat, Hamster (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (0.03 - 0.1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
BHMT antibody was raised against peptide with sequence C-EQQLKELFEKQK, from the C Terminus of the protein sequence according to NP_001704.1 .
Blocking Peptide
LS-E26409 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B3257. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa391-402
Specificity
Human BHMT.
Usage
Immunohistochemistry: LS-B3257 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3257 was determined to be 2.5 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BHMT
Q93088 NM_001713 NP_001704.2

Validation

Anti-BHMT antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC Validated
Anti-BHMT antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-BHMT antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-BHMT antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC Validated
Anti-BHMT antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-BHMT antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Publications (2)

Sulfur amino acid metabolism in doxorubicin-resistant breast cancer cells. Ryu CS, Kwak HC, Lee KS, Kang KW, Oh SJ, Lee KH, Kim HM, Ma JY, Kim SK. Toxicology and applied pharmacology. 2011 255:94-102.

Plasma homocysteine level and hepatic sulfur amino acid metabolism in mice fed a high-fat diet. Yun KU, Ryu CS, Oh JM, Kim CH, Lee KS, Lee CH, Lee HS, Kim BH, Kim SK. European journal of nutrition. 2013 52:127-34. (WB; Mouse)

Reviews (0)

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Requested From: United States
Date Requested: 6/20/2019
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