BHMT antibody LS-B3257 is an unconjugated goat polyclonal antibody to BHMT from human, rat, hamster and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Human, Monkey, Rat, Hamster
(tested or 100% immunogen sequence identity)
- IHC - Paraffin (2.5 µg/ml)
- Western blot (0.03 - 0.1 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
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BHMT antibody was raised against synthetic peptide C-EQQLKELFEKQK from the C-terminus of human BHMT (NP_001704.1 ). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Hamster, Panda (100%); Mouse, Dog, Bat, Bovine, Horse, Pig, Opossum, Chicken (92%); Rat, Elephant, Rabbit (83%).
- Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B3257. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Immunohistochemistry: LS-B3257 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3257 was determined to be 2.5 ug/ml.
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.