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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BHLHE41 / BHLHB3 / SHARP1 Antibody (aa17‑46) LS‑C156808

Catalog Number Size Price
LS-C156808-400 400 µl $325 
Fluorescent confocal image of MCF-7 cell stained with BHLH3 Antibody. MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with BHLH3 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). BHLH3 immunoreactivity is localized to nucleus significantly.
BHLH3 Antibody western blot of RD cell line lysates (35 ug/lane). The BHE41 antibody detected the BHE41 protein (arrow).
BHLH3 Antibody flow cytometry of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Fluorescent confocal image of MCF-7 cell stained with BHLH3 Antibody. MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with BHLH3 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). BHLH3 immunoreactivity is localized to nucleus significantly.
BHLH3 Antibody western blot of RD cell line lysates (35 ug/lane). The BHE41 antibody detected the BHE41 protein (arrow).
BHLH3 Antibody flow cytometry of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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BHLHE41 / BHLHB3 / SHARP1 Antibody (aa17‑46) LS‑C156808

Antibody:
Rabbit Polyclonal to Human BHLHE41 / BHLHB3 / SHARP1
Application:
IF, WB, Flo
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$325 
LS-C156808-400
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human BHLHE41 / BHLHB3 / SHARP1
Application:
IF, WB, Flo
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
SHARP1 antibody LS-C156808 is an unconjugated rabbit polyclonal antibody to SHARP1 (BHLHE41 / BHLHB3 ) from human and mouse. Validated for Flow, IF and WB.
Target
Human BHLHE41 / BHLHB3 / SHARP1
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Blocking Peptide
LS-E7960 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C156808. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa17-46
Specificity
This BHLH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-46 amino acids from the N-terminal region of human BHLH3.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BHLHE41 / BHLHB3 / SHARP1
Q9C0J9 NM_030762 NP_110389.1

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Requested From: United States
Date Requested: 8/17/2019
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