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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BFSP1 / Filensin Antibody (aa405‑665, clone 3C11) LS‑C338979
Filensin antibody LS-C338979 is an unconjugated mouse monoclonal antibody to human Filensin (BFSP1). Validated for IHC and WB.
Catalog
Size
Price
LS-C338979-100
100 µl (1 mg/ml)
$335

Popular BFSP1 / Filensin Products

Antibody
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
IHC of paraffin-embedded Human liver tissue using anti-BFSP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human
Applications: Immunofluorescence, Western blot, ELISA
Antibody
Species: Human
Applications: IHC, IHC - Frozen, Western blot
IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-BFSP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
Species: Human
Applications: IHC, IHC - Paraffin, Western blot

Product Description

Filensin antibody LS-C338979 is an unconjugated mouse monoclonal antibody to human Filensin (BFSP1). Validated for IHC and WB.

Specifications

Target
Human BFSP1 / Filensin
Synonyms
BFSP1, Cytoskeletal protein, 115 KD, CP115, CP94, Filensin, LIFL-H
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [3C11]
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:150)
  • Western blot (1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Human recombinant protein fragment corresponding to amino acids 405-665 of human BFSP1 (NP_001186) produced in E. coli.
Epitope
aa405-665
Specificity
Human BFSP1 / Filensin
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About BFSP1 / Filensin
BFSP1 / Filensin encodes a lens-specific intermediate filament-like protein named filensin. The encoded protein is expressed in lens fiber cells after differentiation has begun. This protein functions as a component of the beaded filament which is a cytoskeletal structure found in lens fiber cells. Mutations in this gene are the cause of autosomal recessive cortical juvenile-onset cataract. Alternate splicing results in multiple transcript variants. Q12934 NM_001195 NP_001186.1


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Images

Immunohistochemistry

IHC of paraffin-embedded Human pancreas tissue using anti-BFSP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
IHC of paraffin-embedded Human pancreas tissue using anti-BFSP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BFSP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BFSP1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BFSP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BFSP1.

Immunohistochemistry

IHC of paraffin-embedded Human pancreas tissue using anti-BFSP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
IHC of paraffin-embedded Human pancreas tissue using anti-BFSP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BFSP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BFSP1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BFSP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BFSP1.

Immunohistochemistry

IHC of paraffin-embedded Human pancreas tissue using anti-BFSP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
IHC of paraffin-embedded Human pancreas tissue using anti-BFSP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BFSP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BFSP1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BFSP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BFSP1.

Immunohistochemistry

IHC of paraffin-embedded Human pancreas tissue using anti-BFSP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
IHC of paraffin-embedded Human pancreas tissue using anti-BFSP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BFSP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BFSP1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BFSP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BFSP1.

Requested From: United States
Date Requested: 11/13/2018

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