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| Catalog Number | Size | Price |
|---|---|---|
| LS-B510-50 | 50 µl (1 mg/ml) | $460 |
IHC‑plus™ Polyclonal Rabbit anti‑Human BECN1 / Beclin‑1 Antibody (IHC, WB) LS‑B510
IHC‑plus™ Polyclonal Rabbit anti‑Human BECN1 / Beclin‑1 Antibody (IHC, WB) LS‑B510
Note: This antibody replaces LS-C2536
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Specifications
Description
Beclin-1 antibody LS-B510 is an unconjugated rabbit polyclonal antibody to Beclin-1 (BECN1) from human. It is reactive with human and mouse. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human BECN1 / Beclin-1
Synonyms
BECN1 | ATG6 | Beclin 1 | Beclin 1, autophagy related | Beclin1 | GT197 | Protein GT197 | VPS30 | Beclin-1
Host
Rabbit
Reactivity
Human, Mouse
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic peptide to human Beclin 1, containing residues within 1-100. This sequence has 94% identity with the mouse sequence and 88% identity with the rat sequence
Specificity
Beclin 1.
Applications
- IHC
- IHC - Paraffin (1.25 µg/ml)
- Western blot (1:2000 - 1:5000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B510 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B510 was determined to be 1.25 ug/ml.
Presentation
TBS, 5 mg/ml BSA, 0.1% Sodium Azide
Storage
Short term: 4°C; Long term: -70°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
About BECN1 / Beclin-1
Validation
Anti-BECN1 / Beclin-1 antibody IHC of human adrenal medulla. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 25 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-BECN1 / Beclin-1 antibody IHC of human adrenal medulla. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 25 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
See More About...
LSBio Ratings
IHC-plus™ BECN1 / Beclin-1 Antibody for IHC, WB/Western LS-B510 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Species:
Human, Mouse, Rat
Applications:
IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Species:
Human, Rat, Dog
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Species:
Human, Mouse
Applications:
IHC, Western blot, Flow Cytometry, ELISA
Species:
Human, Mouse, Rat, Bovine, Pig, Primate
Applications:
IHC, IHC - Paraffin, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/23/2024
Date Requested: 4/23/2024
