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Catalog Number Size Price
LS-C775393-100 100 µg (1 mg/ml) $479 
BECN1 / Beclin-1 Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Beclin 1 Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Beclin 1 Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Beclin 1 Antibody (D) Composite.
BECN1 / Beclin-1 Antibody - Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~51kDa Beclin 1 protein using Rabbit Anti-Beclin 1 Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-Beclin 1 Polyclonal Antibody  at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~51kDa.
BECN1 / Beclin-1 Antibody - Western blot analysis of Rat Liver showing detection of ~51kDa Beclin 1 protein using Rabbit Anti-Beclin 1 Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Rat Liver (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-Beclin 1 Polyclonal Antibody  at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~51kDa.
BECN1 / Beclin-1 Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Beclin 1 Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Beclin 1 Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Beclin 1 Antibody (D) Composite.
BECN1 / Beclin-1 Antibody - Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~51kDa Beclin 1 protein using Rabbit Anti-Beclin 1 Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-Beclin 1 Polyclonal Antibody  at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~51kDa.
BECN1 / Beclin-1 Antibody - Western blot analysis of Rat Liver showing detection of ~51kDa Beclin 1 protein using Rabbit Anti-Beclin 1 Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Rat Liver (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-Beclin 1 Polyclonal Antibody  at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~51kDa.
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Polyclonal Rabbit anti‑Human BECN1 / Beclin‑1 Antibody (Atto 594, IF, WB) LS‑C775393

Polyclonal Rabbit anti‑Human BECN1 / Beclin‑1 Antibody (Atto 594, IF, WB) LS‑C775393

Antibody:
BECN1 / Beclin-1 Rabbit anti-Human Polyclonal (Atto 594) Antibody
Application:
IF, WB
Reactivity:
Human, Rat
Format:
Atto 594, Unmodified
Other formats:
Price
Catalog Number
$479
LS-C775393-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
BECN1 / Beclin-1 Rabbit anti-Human Polyclonal (Atto 594) Antibody
Application:
IF, WB
Reactivity:
Human, Rat
Format:
Atto 594, Unmodified
Other formats:

Specifications

Description
Beclin-1 antibody LS-C775393 is an Atto 594-conjugated rabbit polyclonal antibody to Beclin-1 (BECN1) from human. It is reactive with human and rat. Validated for IF and WB.
Target
Human BECN1 / Beclin-1
Synonyms
BECN1 | ATG6 | Beclin 1 | Beclin 1, autophagy related | Beclin1 | GT197 | Protein GT197 | VPS30 | Beclin-1
Host
Rabbit
Reactivity
Human, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Atto 594. Also available conjugated with AP, APC, Atto 390, Atto 488, Biotin, FITC, PerCP, RPE.
Purification
Peptide Affinity Purified
Modifications
Unmodified
Immunogen
Synthetic peptide from the mid-protein of human Beclin 1
Specificity
Predicted molecular weight at ~51 kDa. Observed a secondary band on some lysates at ~30 kDa.
Applications
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
  • Applications tested for the base form of this product only
Usage
Applications should be user optimized.
Presentation
PBS, 0.09% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BECN1 / Beclin-1
Q14457 NM_003766 NP_003757.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/18/2024