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BECN1 / Beclin‑1 Antibody (aa210‑239) LS‑C156787

Catalog Number Size Price
LS-C156787-400 400 µl $325 
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot of anti-hBECN1-E225 antibody in 293 cell line lysates transiently transfected with the BECN1 gene (2 ug/lane). hBECN1-E225(arrow) was detected using the purified antibody.
The anti-BECN1 antibody is used in Western blot to detect BECN1 in, from left to right, A2058, HeLa, mouse brain, Y79, and HL60 tissue lysates. BECN1(arrow) was detected using the purified antibody.
Flow cytometric of Y79 cells using Beclin 1 (BECN1/APG6) Antibody (E225) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot of anti-hBECN1-E225 antibody in 293 cell line lysates transiently transfected with the BECN1 gene (2 ug/lane). hBECN1-E225(arrow) was detected using the purified antibody.
The anti-BECN1 antibody is used in Western blot to detect BECN1 in, from left to right, A2058, HeLa, mouse brain, Y79, and HL60 tissue lysates. BECN1(arrow) was detected using the purified antibody.
Flow cytometric of Y79 cells using Beclin 1 (BECN1/APG6) Antibody (E225) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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BECN1 / Beclin‑1 Antibody (aa210‑239) LS‑C156787

Antibody:
Rabbit Polyclonal to Human BECN1 / Beclin-1
Application:
IHC, IHC-P, WB, Flo
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$325 
LS-C156787-400
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human BECN1 / Beclin-1
Application:
IHC, IHC-P, WB, Flo
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
Beclin-1 antibody LS-C156787 is an unconjugated rabbit polyclonal antibody to Beclin-1 (BECN1 ) from human and mouse. Validated for Flow, IHC and WB.
Target
Human BECN1 / Beclin-1
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Predicted
Rat, Pig, Cow (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E5661 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C156787. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa210-239
Specificity
This Beclin 1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 210-239 amino acids from human Beclin 1.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BECN1 / Beclin-1
Q14457 NM_003766 NP_003757.1

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Requested From: United States
Date Requested: 8/25/2019
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