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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C156795-400 400 µl $325 
BECN1 / Beclin-1 Antibody - Fluorescent image of U251 cells stained with Beclin 1 antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated Beclin 1 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Beclin1 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
BECN1 / Beclin-1 Antibody - Beclin 1 Antibody western blot of WiDr cell line lysates (35 ug/lane). The Beclin 1 antibody detected the Beclin 1 protein (arrow).
BECN1 / Beclin-1 Antibody - Fluorescent image of U251 cells stained with Beclin 1 antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated Beclin 1 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Beclin1 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
BECN1 / Beclin-1 Antibody - Beclin 1 Antibody western blot of WiDr cell line lysates (35 ug/lane). The Beclin 1 antibody detected the Beclin 1 protein (arrow).
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BECN1 / Beclin‑1 Antibody (aa100‑123) LS‑C156795

BECN1 / Beclin‑1 Antibody (aa100‑123) LS‑C156795

Antibody:
Rabbit Polyclonal to Human BECN1 / Beclin-1
Application:
IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$325 
LS-C156795-400
400 µl
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human BECN1 / Beclin-1
Application:
IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Beclin-1 antibody LS-C156795 is an unconjugated rabbit polyclonal antibody to human Beclin-1 (BECN1 ). Validated for IF and WB.
Target
Human BECN1 / Beclin-1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Pig, Chicken, Xenopus, Cow (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
Blocking Peptide
LS-E2713 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C156795. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa100-123
Specificity
This Beclin 1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 100-123 amino acids from human Beclin 1.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BECN1 / Beclin-1
Q14457 NM_003766 NP_003757.1

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Requested From: United States
Date Requested: 11/21/2019