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Antibodies > BCL2L1 / BCL-XL > LS-C801163
 
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Catalog Number Size Price
LS-C801163-100 100 µl (1 mg/ml) $379 
LS-C801163-200 200 µl (1 mg/ml) $421 
BCL2L1 / BCL-XL Antibody - 1:100 staining human lung carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
BCL2L1 / BCL-XL Antibody - Staining 293 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
BCL2L1 / BCL-XL Antibody - Western blot analysis of BCL-XL phosphorylation expression in UV treated 293 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
BCL2L1 / BCL-XL Antibody - Western blot analysis of Phospho-BCL-XL (Ser62) expression in various lysates
BCL2L1 / BCL-XL Antibody - 1:100 staining human lung carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
BCL2L1 / BCL-XL Antibody - Staining 293 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
BCL2L1 / BCL-XL Antibody - Western blot analysis of BCL-XL phosphorylation expression in UV treated 293 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
BCL2L1 / BCL-XL Antibody - Western blot analysis of Phospho-BCL-XL (Ser62) expression in various lysates
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Polyclonal Rabbit anti‑Human BCL2L1 / BCL‑XL Antibody (phospho‑Ser62, IHC, IF, WB) LS‑C801163

Polyclonal Rabbit anti‑Human BCL2L1 / BCL‑XL Antibody (phospho‑Ser62, IHC, IF, WB) LS‑C801163

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Antibody:
BCL2L1 / BCL-XL Rabbit anti-Human Polyclonal (pSer62) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C801163-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
BCL2L1 / BCL-XL Rabbit anti-Human Polyclonal (pSer62) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
BCL-XL antibody LS-C801163 is an unconjugated rabbit polyclonal antibody to BCL-XL (BCL2L1) (pSer62) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human BCL2L1 / BCL-XL
Synonyms
BCL2L1 | Apoptosis regulator Bcl-X | Bcl-xL | BCL-XL/S | BCLXS | Bcl-2-like protein 1 | BCL2-like 1 | BCL2L | Bcl-X | Bcl-xS | BCLXL | PPP1R52 | Bcl2-L-1 | BCLX
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human BCL-XL around the phosphorylation site of Serine 62.
Epitope
pSer62
Specificity
Phospho-BCL-XL (Ser62) Antibody detects endogenous levels of BCL-XL only when phosphorylated at Serine 62.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BCL2L1 / BCL-XL
Q07817 NM_001191 NP_001182.1

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Species: Human, Mouse, Rat, Dog, Hamster, Pig
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BCL2L1 / BCL-XL Antibody - IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-BCL2L1 mouse monoclonal antibody.
BCL2L1 / BCL‑XL Antibody (clone OTI2D1) LS‑C174729
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
BCL2L1 / BCL-XL Antibody - Western blot of lysates from A431, HeLa, HepG2, Jurkat, rat PC-12 cell line (from left to right) with BCL2L1 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.
BCL2L1 / BCL‑XL Antibody (aa195‑229) LS‑C344429
Species: Human, Rat
Applications: Western blot
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Species: Human
Applications: Western blot, ELISA
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Species: Human
Applications: IHC, Western blot, Immunoprecipitation, Flow Cytometry
See all BCL2L1 / BCL-XL Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
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