Human BCL2L1 / BCL-XL

Mouse

Human, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Sheep (tested or 100% immunogen sequence identity)

IgG2a Monoclonal

BX006 + 2H12

Unconjugated

Protein G purified

Azide-free, BSA-free. Also available Unmodified or Ready-to-Use.

Synthetic peptide (QSNRELVVDFLS, aa3-14 of UniProt Q07817) from the human protein. Percent identity by BLAST analysis: Human, Mouse, Rat, Ferret, Sheep, Cat, Bovine, Bat, Hamster, Elephant, Rabbit, Horse, Pig, Dog (100%); Guinea pig (92%); Opossum, Lizard (83%).

aa3-14

Human BCL2L1 / Bcl-xL

• IHC
• IHC - Paraffin (0.5 - 1 µg/ml)
• Immunofluorescence (0.5 - 1 µg/ml)
• Western blot (0.5 - 1 µg/ml)
• Flow Cytometry (0.5 - 1 µg/10E6 cells)

The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Recognizes a protein of 27kDa, identified as the Bcl-X protein. This mAb cocktail shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein which suppresses cell death. And Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2. This mAb reacts with both Bcl-XS and Bcl-XL proteins. The optimal dilution of the Bcl-X antibody for each application should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.

1X PBS

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q07817 NCBI: NM_001191 NP_001182.1