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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BCKDHA / BCKDE1A Antibody (phospho-Ser292) for WB/Western LS-C762191

Catalog
Size
Price
LS-C762191-100
100 µg (1 mg/ml)
$415
Target
Mouse BCKDHA / BCKDE1A
Host
Rabbit
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Predicted
Human, Chimpanzee, Rat, Cow (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • Western blot (1:2000 - 1:10000)
Immunogen
Portion of GeneID: 593 corresponding to ammino acids surrounding phospho-serine 292 of UniprotID: P12694
Epitope
pSer292
Usage
Applications should be user optimized.
Manufacturer
Bethyl Laboratories, Inc.
Presentation
Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
Storage
Store at 2°C to 8°C for up to 1 year.
Restrictions
For research use only.
About BCKDHA / BCKDE1A
P12694 NM_000709 NP_000700.1

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Images

Western blot

Detection of mouse Phospho BCKDHA (S292) by western blot. Samples: Whole cell lysate (50 µg) from differentiated 3T3-L1 (3T3L1d) and 3T3-L1 (3T3L1) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Phospho BCKDHA (S292) antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Detection of mouse Phospho BCKDHA (S292) by western blot. Samples: Whole cell lysate (50 µg) from differentiated 3T3-L1 (3T3L1d) and 3T3-L1 (3T3L1) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Phospho BCKDHA (S292) antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Western blot

Detection of mouse Phospho BCKDHA (S292) by western blot. Samples: Whole cell lysate (50 µg) from differentiated 3T3-L1 (3T3L1d) and 3T3-L1 (3T3L1) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Phospho BCKDHA (S292) antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Detection of mouse Phospho BCKDHA (S292) by western blot. Samples: Whole cell lysate (50 µg) from differentiated 3T3-L1 (3T3L1d) and 3T3-L1 (3T3L1) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Phospho BCKDHA (S292) antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Western blot

Detection of mouse Phospho BCKDHA (S292) by western blot. Samples: Whole cell lysate (50 µg) from differentiated 3T3-L1 (3T3L1d) and 3T3-L1 (3T3L1) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Phospho BCKDHA (S292) antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Detection of mouse Phospho BCKDHA (S292) by western blot. Samples: Whole cell lysate (50 µg) from differentiated 3T3-L1 (3T3L1d) and 3T3-L1 (3T3L1) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Phospho BCKDHA (S292) antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Western blot

Detection of mouse Phospho BCKDHA (S292) by western blot. Samples: Whole cell lysate (50 µg) from differentiated 3T3-L1 (3T3L1d) and 3T3-L1 (3T3L1) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Phospho BCKDHA (S292) antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Detection of mouse Phospho BCKDHA (S292) by western blot. Samples: Whole cell lysate (50 µg) from differentiated 3T3-L1 (3T3L1d) and 3T3-L1 (3T3L1) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Phospho BCKDHA (S292) antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Requested From: United States
Date Requested: 5/19/2019
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