BAP31 antibody LS-B2117 is an unconjugated rat monoclonal antibody to BAP31 (BCAP31). Validated for ELISA, ICC, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Human BCAP31 / BAP31
- IHC - Paraffin (2.5 µg/ml)
- IHC - Frozen (1:1000 - 1:10000)
- ICC (1:1000 - 1:10000)
- Western blot (1:1000 - 1:6000)
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BCAP31 / BAP31 antibody was raised against fusion protein from human BCAP31 / BAP31.
Recognizes an epitope (aa 230-246) encompassing a caspase recognition site and the ER-homing motif (KKEE), present at the C-terminus of human, primate, bovine and hamster BAP31. Does not recognize mouse and rat BAP31.
Immunohistochemistry: LS-B2117 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2117 was determined to be 2.5 ug/ml.
PBS, pH 7.2, 0.02% Sodium Azide
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
For research use only.