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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BCAP31 / BAP31 Antibody (clone CC‑1) IHC‑plus™ LS‑B2117
Note: This antibody replaces LS-C18699
BAP31 antibody LS-B2117 is an unconjugated rat monoclonal antibody to BAP31 (BCAP31). Validated for ELISA, ICC, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B2117-50
50 µg
Unavailable

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Product Description

BAP31 antibody LS-B2117 is an unconjugated rat monoclonal antibody to BAP31 (BCAP31). Validated for ELISA, ICC, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
About BCAP31 / BAP31
Functions as a chaperone protein. Is one of the most abundant endoplasmic reticulum (ER) proteins. Plays a role in the export of secreted proteins in the ER, the recognition of abnormally folded protein and their targeting to the ER associated-degradation (ERAD). Also serves as a cargo receptor for the export of transmembrane proteins. May be involved in CASP8-mediated apoptosis. P51572 NM_005745 NP_005736.3

BCAP31 Antibody, 6C6-AG Antibody, BCR-associated protein 31 Antibody, BAP31 Antibody, BCR-associated protein Bap31 Antibody, DXS1357E Antibody, p28 Bap31 Antibody, CDM Antibody, p28 Antibody, Protein CDM Antibody


Specifications

Target
Human BCAP31 / BAP31
Host
Rat
Clonality
IgG2a Monoclonal [CC-1]
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • IHC - Frozen (1:1000 - 1:10000)
  • ICC (1:1000 - 1:10000)
  • Western blot (1:1000 - 1:6000)
  • Immunoprecipitation
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
BCAP31 / BAP31 antibody was raised against fusion protein from human BCAP31 / BAP31.
Specificity
Recognizes an epitope (aa 230-246) encompassing a caspase recognition site and the ER-homing motif (KKEE), present at the C-terminus of human, primate, bovine and hamster BAP31. Does not recognize mouse and rat BAP31.
Usage
Immunohistochemistry: LS-B2117 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2117 was determined to be 2.5 ug/ml.
Presentation
PBS, pH 7.2, 0.02% sodium azide.
Storage
4°C or -20°C, Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Immunohistochemistry

Anti-BCAP31 / BAP31 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2117 concentration 5 ug/ml.
Anti-BCAP31 / BAP31 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2117 concentration 5 ug/ml.

Immunohistochemistry

Anti-BCAP31 / BAP31 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2117 concentration 5 ug/ml.
Anti-BCAP31 / BAP31 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2117 concentration 5 ug/ml.

Immunohistochemistry

Anti-BCAP31 / BAP31 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2117 concentration 5 ug/ml.
Anti-BCAP31 / BAP31 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2117 concentration 5 ug/ml.

Immunohistochemistry

Anti-BCAP31 / BAP31 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2117 concentration 5 ug/ml.
Anti-BCAP31 / BAP31 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2117 concentration 5 ug/ml.


Requested From: 
Date Requested: 6/17/2018

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