Human BBC3 / PUMA

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Purified

Unmodified

Synthetic peptide ARARQEGSSPEPVEG corresponding to the N-Terminus of human PUMA alpha. Percent identity by BLAST analysis: Human, Gibbon, Mouse, Rat, Porcine (100%); Opossum (87%).

aa2-16

Recognizes the p53 - upregulated modulator of apoptosis, also known as Bcl-2 binding component 3, JFY-1 or Bcl-2. PUMA is a 193 amino acid ~20 kD pro-apoptotic member of the BCL-2 family. Bcl-2 binding component 3 is localized to the mitochondria, binds BCL-2 and induces cytochrome C release and apoptosis in response to p53. Four isoforms of PUMA have been described due to alternative splicing. Is expected to recognize the canonical isofom 1, also known as PUMA alpha, it is not expected to recognize any of the other isoforms of PUMA.

• IHC
• IHC - Paraffin (2.5 µg/ml)
• Western blot (2 - 4 µg/ml)

Immunohistochemistry: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose. Histology Positive Control Tissue: Human breast carcinoma. Western Blot: detects a band of approximately 23kD in K562 cell lysates.

PBS, 0.02% Sodium Azide

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q9BXH1 NCBI: NM_014417 NP_055232.1