Human BAX

Mouse

Human, Monkey, Bovine, Dog, Goat, Pig (tested or 100% immunogen sequence identity)

Mouse, Rat

IgG1 Monoclonal

SPM336

Unconjugated

Protein G purified

Azide-free, BSA-free. Also available Unmodified.

Synthetic peptide (GSGEQPRGGGPTSS, aa3-16 of UniProt Q07812) from the human protein. Percent identity by BLAST analysis: Human, Ferret, Cat, Bovine, Goat, Pig, Dog (100%); Bat, Elephant, Opossum (93%); Rat, Hamster, Guinea pig (86%); Mouse (79%).

aa3-16

Human BAX

• IHC
• IHC - Paraffin (0.5 - 1 µg/ml)
• Immunofluorescence (1 - 2 µg/ml)
• Western blot (0.5 - 1 µg/ml)
• Flow Cytometry (0.5 - 1 µg/10E6 cells)

The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Recognizes a protein of 21kDa, identified as the Bax protein. This mAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2. The optimal dilution of the anti-Bax antibody for each application should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.

1X PBS

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q07812 NCBI: NM_004324 NP_004315.1