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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C391493-20 20 µg (0.2 mg/ml) $295 
LS-C391493-100 100 µg (0.2 mg/ml) $465 
Basic Cytokeratin Antibody - Formalin-fixed, paraffin-embedded human prostate carcinoma stained with Basic Cytokeratin antibody (SPM591).  This image was taken for the unmodified form of this product. Other forms have not been tested.

Basic Cytokeratin Antibody (clone SPM591) LS‑C391493

Basic Cytokeratin Antibody (clone SPM591) LS‑C391493

Antibody:
Mouse Monoclonal to Human Basic Cytokeratin
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$295 
LS-C391493-20
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human Basic Cytokeratin
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
Basic Cytokeratin antibody LS-C391493 is an unconjugated mouse monoclonal antibody to Basic Cytokeratin from human, mouse and rat. Validated for Flow, IF, IHC and WB.
Target
Human Basic Cytokeratin
Host
Mouse
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
SPM591
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified. Also available Azide-free.
Applications
  • IHC
  • IHC - Paraffin (0.25 - 0.5 µg/ml)
  • Immunofluorescence (0.5 - 1 µg/ml)
  • Western blot (0.5 - 1 µg/ml)
  • Flow Cytometry (0.5 - 1 µg/10E6 cells)
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Solubilized keratin extract from human stratum corneum.
Specificity
Human Basic Cytokeratin
Usage
This antibody recognizes CK1, CK5, CK10 and CK14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. This mAb shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands. there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this antibody. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. This antibody has also been useful in separating benign from malignant intraductal breast proliferations. Optimal dilution of the Basic Cytokeratin antibody should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Presentation
0.01% BSA, 0.05% sodium azide
Storage
Store at 2°C to 8°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Basic Cytokeratin Antibody - Formalin paraffin rat oviduct with Basic Cytokeratin antibody (KRTH/1076).  This image was taken for the unmodified form of this product. Other forms have not been tested.
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Basic Cytokeratin Antibody - Formalin paraffin rat oviduct with Basic Cytokeratin antibody (KRTH/1076).  This image was taken for the unmodified form of this product. Other forms have not been tested.
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Basic Cytokeratin Antibody - Formalin-fixed, paraffin-embedded human prostate carcinoma stained with Basic Cytokeratin antibody (SPM591).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
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LPA / Lipoprotein a ELISA Kit
Reactivity: Monkey
Range: 31.25-2000 ng/ml

Requested From: United States
Date Requested: 10/22/2019