Human Basic Cytokeratin

Mouse

Human, Rat (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

KRTH/1076

Unconjugated

Protein G purified

Ready-to-Use. Also available Unmodified or Azide-free, BSA-free.

Recombinant human KRT76 protein.

Human Basic Cytokeratin

• IHC
• IHC - Paraffin (1:1)
• Immunofluorescence
• Western blot
• ELISA

The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. This mAb recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1). 64kDa (CK3). 59kDa (CK4). 58kDa (CK5). 56kDa (CK6). 52kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. Optimal dilution of the Basic Cytokeratin antibody should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.