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Catalog Number Size Price
LS-B8289-50 50 µg (0.5 mg/ml) $485 
BAIAP2 / IRSP53 Antibody - Anti-BAIAP2 / IRSP53 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
BAIAP2 / IRSP53 Antibody - HEK293 lysate (10ug protein in RIPA buffer) over expressing Human BAIAP2 with DYKDDDDK tag probed with (1.0 ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Pri
BAIAP2 / IRSP53 Antibody - BAIAP2 antibody staining (1 ug/ml) of Human Brain (Cerebellum) lysate (RIPA buffer, 35g total protein per lane). Primary incubated for 12 hour. Detected by Western blot of chemiluminescence.
BAIAP2 / IRSP53 Antibody - Anti-BAIAP2 / IRSP53 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
BAIAP2 / IRSP53 Antibody - HEK293 lysate (10ug protein in RIPA buffer) over expressing Human BAIAP2 with DYKDDDDK tag probed with (1.0 ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Pri
BAIAP2 / IRSP53 Antibody - BAIAP2 antibody staining (1 ug/ml) of Human Brain (Cerebellum) lysate (RIPA buffer, 35g total protein per lane). Primary incubated for 12 hour. Detected by Western blot of chemiluminescence.
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IHC‑plus™ Polyclonal Goat anti‑Human BAIAP2 / IRSP53 Antibody (Isoform 3, IHC, WB) LS‑B8289

IHC‑plus™ Polyclonal Goat anti‑Human BAIAP2 / IRSP53 Antibody (Isoform 3, IHC, WB) LS‑B8289

Note: This antibody replaces LS-C34628
Antibody:
BAIAP2 / IRSP53 Goat anti-Human Polyclonal (Isoform 3) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-B8289-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
BAIAP2 / IRSP53 Goat anti-Human Polyclonal (Isoform 3) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
IRSP53 antibody LS-B8289 is an unconjugated goat polyclonal antibody to human IRSP53 (BAIAP2) (Isoform 3). Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human BAIAP2 / IRSP53
Synonyms
BAIAP2 | BAI-associated protein 2 | BAI1-associated protein 2 | BAP2 | FLAF3 | Fas ligand-associated factor 3 | IRSP53 | IRS-58 | Protein BAP2 | IRSp53/58
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-SMSSADVEVARF, from the C-Terminus of protein sequence according to NP_006331.1.
Epitope
Isoform 3
Specificity
Human BAIAP2 / IRSP53. This antibody will recognise only one of three reported isoforms (NP_006331.1).
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Western blot (1 - 2 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:8000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 55 kDa band observed in Human Brain (Cerebellum and Frontal Cortex) lysates (calculated MW of 57.4 kDa according to NP_006331.1). Recommended concentration: 1-3 ug/ml. An additional band of unknown identity was also consistently observed at 150-170 kDa. This band was successfully blocked by incubation with the immunising peptide.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BAIAP2 / IRSP53
Q9UQB8 NM_017450 NP_059344.1

Validation

BAIAP2 / IRSP53 Antibody - Anti-BAIAP2 / IRSP53 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
Anti-BAIAP2 / IRSP53 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
Anti-BAIAP2 / IRSP53 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.

LSBio Ratings

IHC-plus™ BAIAP2 / IRSP53 Antibody (Isoform 3) for IHC, WB/Western LS-B8289 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/18/2024