BAD antibody LS-C3909 is an unconjugated rabbit polyclonal antibody to BAD from human and mouse. Validated for ELISA and WB.
(tested or 100% immunogen sequence identity)
Protein A purified
- Western blot (1:1000)
- ELISA (1:1000)
BAD antibody was raised against synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser155 of mouse Bad.
Detects overexpressed levels of Bad only when phosphorylated at Ser155. Does not detect Bad phosphorylated at other sites or related family members. Species cross-reactivity: Human.
Suitable for use in ELISA and Western Blot. Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight. ELISA: 1:1000.
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol, No preservatives added
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.