Target
Mouse BAD
Synonyms
BAD | BBC2 | Bcl-2-like protein 8 | BCL2-binding component 6 | BCL2L8 | BBC6 | BCL-X/BCL-2 binding protein | Bcl-2-binding component 6 | Bcl2 antagonist of cell death | BCL2-binding protein | Bcl2-L-8
Reactivity
Mouse, Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Protein A purified
Immunogen
Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser155 of mouse Bad.
Specificity
Detects overexpressed levels of Bad only when phosphorylated at Ser155. Does not detect Bad phosphorylated at other sites or related family members. Species cross-reactivity: Human.
Applications
- Western blot (1:1000)
- ELISA (1:1000)
Usage
Suitable for use in ELISA and Western Blot. Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight. ELISA: 1:1000.
Presentation
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol, No preservatives added
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee