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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BAD Antibody (phospho‑Ser136) LS‑C205248
BAD antibody LS-C205248 is an unconjugated rabbit polyclonal antibody to BAD from human and mouse. Validated for IHC and WB.
Catalog
Size
Price
LS-C205248-50
50 µl (1 mg/ml)
$355
Description
BAD antibody LS-C205248 is an unconjugated rabbit polyclonal antibody to BAD from human and mouse. Validated for IHC and WB.
Target
Mouse BAD
Host
Rabbit
Reactivity
Mouse, Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
BAD antibody was raised against peptide sequence around phosphorylation site of serine136 (S-R-S (p) -A-P) of Mouse BAD.
Epitope
pSer136
Specificity
The antibody detects endogenous levels of BAD only when phosphorylated at serine136.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About BAD
Q92934 NM_004322 NP_004313.1

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Images

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human breast carcinoma tissue using BAD (Phospho-Ser136) antibody (left) or the same antibody preincubated with blocking peptide (right).
Immunohistochemistry of paraffin-embedded human breast carcinoma tissue using BAD (Phospho-Ser136) antibody (left) or the same antibody preincubated with blocking peptide (right).

Western blot

Western blot of extracts from HT29 cells untreated or treated with UV using BAD (Phospho-Ser136) antibody.
Western blot of extracts from HT29 cells untreated or treated with UV using BAD (Phospho-Ser136) antibody.

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human breast carcinoma tissue using BAD (Phospho-Ser136) antibody (left) or the same antibody preincubated with blocking peptide (right).
Immunohistochemistry of paraffin-embedded human breast carcinoma tissue using BAD (Phospho-Ser136) antibody (left) or the same antibody preincubated with blocking peptide (right).

Western blot

Western blot of extracts from HT29 cells untreated or treated with UV using BAD (Phospho-Ser136) antibody.
Western blot of extracts from HT29 cells untreated or treated with UV using BAD (Phospho-Ser136) antibody.

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human breast carcinoma tissue using BAD (Phospho-Ser136) antibody (left) or the same antibody preincubated with blocking peptide (right).
Immunohistochemistry of paraffin-embedded human breast carcinoma tissue using BAD (Phospho-Ser136) antibody (left) or the same antibody preincubated with blocking peptide (right).

Western blot

Western blot of extracts from HT29 cells untreated or treated with UV using BAD (Phospho-Ser136) antibody.
Western blot of extracts from HT29 cells untreated or treated with UV using BAD (Phospho-Ser136) antibody.

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human breast carcinoma tissue using BAD (Phospho-Ser136) antibody (left) or the same antibody preincubated with blocking peptide (right).
Immunohistochemistry of paraffin-embedded human breast carcinoma tissue using BAD (Phospho-Ser136) antibody (left) or the same antibody preincubated with blocking peptide (right).

Western blot

Western blot of extracts from HT29 cells untreated or treated with UV using BAD (Phospho-Ser136) antibody.
Western blot of extracts from HT29 cells untreated or treated with UV using BAD (Phospho-Ser136) antibody.

Requested From: United States
Date Requested: 12/13/2018
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