BAD antibody LS-B3293 is an unconjugated mouse monoclonal antibody to BAD from human and mouse. Validated for IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein G purified
- IHC - Paraffin (10 µg/ml)
- Western blot
- (applications tested for the base form of this product only)
See our complete line of Immunohistochemistry Reagents
including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
BAD antibody was raised against full-length murine Bad containing a N-terminal His6-tag.
Recognizes human Bad, Mr 23kD, and mouse Bad, Mr 31kD. Occasionally a Mr 14kD protein is detected in human lysates.
Immunohistochemistry: LS-B3293 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3293 was determined to be 10 ug/ml. Western Blot: Proteins were visualized using IgG (HRP) goat anti-mouse and a chemiluminescence detection system. The endogenous level of Bad in most lysates is below the detectable limits of this antibody. Immunoprecipitation: 4 ug immunoprecipitates Bad which was subsequently detected using anti-phospho Bad.
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.