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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BACE1 / BACE Antibody LS‑C3879
BACE antibody LS-C3879 is an unconjugated mouse monoclonal antibody to human BACE (BACE1). Validated for ELISA, Flow and WB.
Antibody
Catalog
Size
Price
LS-C3879-100
100 µg
$635
LS-C3879-500
500 µg (0.5 mg/ml)
$1,380

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Product Description

BACE antibody LS-C3879 is an unconjugated mouse monoclonal antibody to human BACE (BACE1). Validated for ELISA, Flow and WB.

Specifications

Target
Human BACE1 / BACE
Synonyms
BACE1, APP beta-secretase, Asp 2, ASP2, Aspartyl protease 2, Aspartylprotease 2, Beta-secretase, Beta-site APP-cleaving enzyme, BACE, HSPC104, KIAA1149, B-Secretase, Beta-secretase 1, Memapsin-2
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • Western blot (1:10000)
  • Flow Cytometry
  • ELISA
Immunogen
BACE1 / BACE antibody was raised against synthetic peptide from human BACE1.
Specificity
Recognizes human BACE-1. This antibody was selected for its ability to detect human or mouse BACE-1 in Western Blots and direct ELISAs. In Western Blots, no cross-reactivity was observed with rhBACE-2, rmBACE-2, rhADAM8, rmADAM9, rmADAM10, rhADAM15, or rhTACE. Species cross-reactivity: Mouse.
Usage
Western Blot-This antibody can be used at 1-2 ug/mL with the appropriate secondary reagents to detect human or mouse BACE-1. Using a colorimetric detection system, the detection limit for rhBACE-1 is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection with Chemiluminescent Detection Substrate will increase sensitivity by 5 to 50 fold. Flow Cytometry-Dilute this antibody to 25 ug/mL and add 10 ul of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 ul of a 25 ug/mL stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Direct ELISA-This antibody can be used at 0.5-1.0 ug/mL with the appropriate secondary reagents to detect human or mouse BACE-1 coated on 96-well plates. The detection limit for rhBACE-1 is approximately 1 ng/well.
Presentation
Lyophilized from PBS, 5% Trehalose
Reconstitution
1,000 µl Reconstitute in 1 ml of Sterile PBS.
Storage
Store at -20°C prior to reconstitution. After reconstitution aliquot and freeze at-20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About BACE1 / BACE
Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. P56817 NM_012104 NP_036236.1


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Requested From: United States
Date Requested: 10/19/2018

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