Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(tested or 100% immunogen sequence identity)
Protein G purified
Western blot (1:10000)
BACE1 / BACE antibody was raised against synthetic peptide from human BACE1.
Recognizes human BACE-1. This antibody was selected for its ability to detect human or mouse BACE-1 in Western Blots and direct ELISAs. In Western Blots, no cross-reactivity was observed with rhBACE-2, rmBACE-2, rhADAM8, rmADAM9, rmADAM10, rhADAM15, or rhTACE. Species cross-reactivity: Mouse.
Western Blot-This antibody can be used at 1-2 ug/mL with the appropriate secondary reagents to detect human or mouse BACE-1. Using a colorimetric detection system, the detection limit for rhBACE-1 is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection with Chemiluminescent Detection Substrate will increase sensitivity by 5 to 50 fold. Flow Cytometry-Dilute this antibody to 25 ug/mL and add 10 ul of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 ul of a 25 ug/mL stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Direct ELISA-This antibody can be used at 0.5-1.0 ug/mL with the appropriate secondary reagents to detect human or mouse BACE-1 coated on 96-well plates. The detection limit for rhBACE-1 is approximately 1 ng/well.
Lyophilized from PBS, 5% Trehalose
1,000 µl Reconstitute in 1 ml of Sterile PBS.
Store at -20°C prior to reconstitution. After reconstitution aliquot and freeze at-20°C. Avoid freeze-thaw cycles.
Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase.