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Catalog Number Size Price
LS-C3879-100 100 µg $680 
LS-C3879-500 500 µg $1,410 
Antibody

Monoclonal BACE1 / BACE Antibody (WB) LS‑C3879

Monoclonal BACE1 / BACE Antibody (WB) LS‑C3879

Antibody:
Mouse Monoclonal to Human BACE1 / BACE
Application:
WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$680 
LS-C3879-100
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human BACE1 / BACE
Application:
WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
BACE antibody LS-C3879 is an unconjugated mouse monoclonal antibody to human BACE (BACE1 ). Validated for ELISA, Flow and WB.
Target
Human BACE1 / BACE
Synonyms
BACE1 | APP beta-secretase | Asp 2 | ASP2 | Aspartyl protease 2 | Aspartylprotease 2 | Beta-secretase | Beta-site APP-cleaving enzyme | BACE | HSPC104 | KIAA1149 | B-Secretase | Beta-secretase 1 | Memapsin-2
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • Western blot (1:10000)
  • Flow Cytometry
  • ELISA
Immunogen
Synthetic peptide from human BACE1.
Specificity
Recognizes human BACE-1. This antibody was selected for its ability to detect human or mouse BACE-1 in Western Blots and direct ELISAs. In Western Blots, no cross-reactivity was observed with rhBACE-2, rmBACE-2, rhADAM8, rmADAM9, rmADAM10, rhADAM15, or rhTACE. Species cross-reactivity: Mouse.
Usage
Western Blot-This antibody can be used at 1-2 ug/mL with the appropriate secondary reagents to detect human or mouse BACE-1. Using a colorimetric detection system, the detection limit for rhBACE-1 is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection with Chemiluminescent Detection Substrate will increase sensitivity by 5 to 50 fold. Flow Cytometry-Dilute this antibody to 25 ug/mL and add 10 ul of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 ul of a 25 ug/mL stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Direct ELISA-This antibody can be used at 0.5-1.0 ug/mL with the appropriate secondary reagents to detect human or mouse BACE-1 coated on 96-well plates. The detection limit for rhBACE-1 is approximately 1 ng/well.
Presentation
Lyophilized from PBS, 5% Trehalose
Reconstitution
Reconstitute with 1 ml sterile PBS.
Storage
Store at -20°C prior to reconstitution. After reconstitution aliquot and freeze at-20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BACE1 / BACE
P56817 NM_012104 NP_036236.1

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Requested From: United States
Date Requested: 2/19/2020