BACE antibody LS-C3879 is an unconjugated mouse monoclonal antibody to human BACE (BACE1). Validated for ELISA, Flow and WB.
Human BACE1 / BACE
(tested or 100% immunogen sequence identity)
Protein G purified
- Western blot (1:10000)
- Flow Cytometry
BACE1 / BACE antibody was raised against synthetic peptide from human BACE1.
Recognizes human BACE-1. This antibody was selected for its ability to detect human or mouse BACE-1 in Western Blots and direct ELISAs. In Western Blots, no cross-reactivity was observed with rhBACE-2, rmBACE-2, rhADAM8, rmADAM9, rmADAM10, rhADAM15, or rhTACE. Species cross-reactivity: Mouse.
Western Blot-This antibody can be used at 1-2 ug/mL with the appropriate secondary reagents to detect human or mouse BACE-1. Using a colorimetric detection system, the detection limit for rhBACE-1 is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection with Chemiluminescent Detection Substrate will increase sensitivity by 5 to 50 fold. Flow Cytometry-Dilute this antibody to 25 ug/mL and add 10 ul of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 ul of a 25 ug/mL stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Direct ELISA-This antibody can be used at 0.5-1.0 ug/mL with the appropriate secondary reagents to detect human or mouse BACE-1 coated on 96-well plates. The detection limit for rhBACE-1 is approximately 1 ng/well.
Lyophilized from PBS, 5% Trehalose
Reconstitute in 1 ml of Sterile PBS.
Store at -20°C prior to reconstitution. After reconstitution aliquot and freeze at-20°C. Avoid freeze-thaw cycles.
For research use only.