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Catalog Number Size Price
LS-C744966-25 25 µl (1 mg/ml) $275 
B2M / Beta 2 Microglobulin Antibody - Immunohistochemistry of rabbit anti-Beta-2-Microglobulin Antibody. Tissue: normal human skin. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Left panel: isotype control, Right panel: ß2 microglobulin antibody at 1 ug/ml for 20 min at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: ß2 microglobulin is cell membrane (and to lesser amount cytoplasmatic compartment). Staining: Beta-2-Microglobulin as brown with diaminobenzidine and with a hematoxylin purple counterstain.
B2M / Beta 2 Microglobulin Antibody - Immunofluorescence Microscopy of rabbit Anti-Beta-2-microglobulin antibody. Tissue: Human Embryonic Kidney cells (HEK 293 cells). Fixation: 2% paraformaldehyde for 15 minutes. Antigen retrieval: not required. Primary antibody: ß-2 microglobulin antibody at 1:200 for overnight at 4°C. Secondary antibody: donkey anti-rabbit secondary antibody conjugated with Alexa Fluor 488 fluorochrome at 1:1,000 for 45 min at RT. Localization: ß2-microglobulin is mainly on the cell surface and occasionally cytoplasmic. Staining: Beta-2-microglobulin as green fluorescent signal.
B2M / Beta 2 Microglobulin Antibody - Western Blot of rabbit Anti-Beta-2-Microglobulin antibody. Lane 1: Beta-2-Microglobulin. Lane 2: None. Load: 50 ng per lane. Primary antibody: Beta-2-Microglobulin primary antibody at 1:1,000 overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 12 kDa, 12 kDa for Beta-2-Microglobulin. Other band(s): Beta-2-Microglobulin splice variants and isoforms.
B2M / Beta 2 Microglobulin Antibody - Immunohistochemistry of rabbit anti-Beta-2-Microglobulin Antibody. Tissue: normal human skin. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Left panel: isotype control, Right panel: ß2 microglobulin antibody at 1 ug/ml for 20 min at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: ß2 microglobulin is cell membrane (and to lesser amount cytoplasmatic compartment). Staining: Beta-2-Microglobulin as brown with diaminobenzidine and with a hematoxylin purple counterstain.
B2M / Beta 2 Microglobulin Antibody - Immunofluorescence Microscopy of rabbit Anti-Beta-2-microglobulin antibody. Tissue: Human Embryonic Kidney cells (HEK 293 cells). Fixation: 2% paraformaldehyde for 15 minutes. Antigen retrieval: not required. Primary antibody: ß-2 microglobulin antibody at 1:200 for overnight at 4°C. Secondary antibody: donkey anti-rabbit secondary antibody conjugated with Alexa Fluor 488 fluorochrome at 1:1,000 for 45 min at RT. Localization: ß2-microglobulin is mainly on the cell surface and occasionally cytoplasmic. Staining: Beta-2-microglobulin as green fluorescent signal.
B2M / Beta 2 Microglobulin Antibody - Western Blot of rabbit Anti-Beta-2-Microglobulin antibody. Lane 1: Beta-2-Microglobulin. Lane 2: None. Load: 50 ng per lane. Primary antibody: Beta-2-Microglobulin primary antibody at 1:1,000 overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 12 kDa, 12 kDa for Beta-2-Microglobulin. Other band(s): Beta-2-Microglobulin splice variants and isoforms.
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Polyclonal Rabbit anti‑Human B2M / Beta 2 Microglobulin Antibody (Liquid, IHC, IF, WB) LS‑C744966

Polyclonal Rabbit anti‑Human B2M / Beta 2 Microglobulin Antibody (Liquid, IHC, IF, WB) LS‑C744966

Antibody:
B2M / Beta 2 Microglobulin Rabbit anti-Human Polyclonal (Liquid) Antibody
Application:
IHC, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Liquid
Price
Catalog Number
$275
LS-C744966-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
B2M / Beta 2 Microglobulin Rabbit anti-Human Polyclonal (Liquid) Antibody
Application:
IHC, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Liquid

Specifications

Description
Beta 2 Microglobulin antibody LS-C744966 is an unconjugated rabbit polyclonal antibody to human Beta 2 Microglobulin (B2M). Validated for ELISA, IF, IHC and WB.
Target
Human B2M / Beta 2 Microglobulin
Synonyms
B2M | Beta 2 Microglobulin | Beta-2-microglobulin | Beta-2-microglobin
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Liquid
Immunogen
Anti-Beta-2-Microglobulin Antibody was produced by repeated immunizations with human beta-2-Microglobulin protein isolated from urine.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified b2-Microglobulin [Human Urine]. Cross reactivity against b2-Microglobulin from other sources is unknown.
Applications
  • IHC (1:500 - 1:2000)
  • Immunofluorescence (1:200)
  • Western blot (1:1000 - 1:5000)
  • ELISA (1:2000 - 1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About B2M / Beta 2 Microglobulin
P61769 NM_004048 NP_004039.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/17/2021