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Catalog Number Size Price
LS-C775395-100 100 µg (1 mg/ml) $479 
AUTL1 / ATG4C Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4C Polyclonal Antibody. Tissue: Myoblast cell line (C2C12). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4C Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG4C Antibody (D) Composite.
AUTL1 / ATG4C Antibody - Western blot analysis of Rat Liver showing detection of ~52.5kDa ATG4C protein using Rabbit Anti-ATG4C Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Rat Liver (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG4C Polyclonal Antibody  at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~52.5kDa.
AUTL1 / ATG4C Antibody - Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~52.5kDa ATG4C protein using Rabbit Anti-ATG4C Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Human 293T (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG4C Polyclonal Antibody  at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~52.5kDa.
AUTL1 / ATG4C Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4C Polyclonal Antibody. Tissue: Myoblast cell line (C2C12). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4C Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG4C Antibody (D) Composite.
AUTL1 / ATG4C Antibody - Western blot analysis of Rat Liver showing detection of ~52.5kDa ATG4C protein using Rabbit Anti-ATG4C Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Rat Liver (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG4C Polyclonal Antibody  at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~52.5kDa.
AUTL1 / ATG4C Antibody - Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~52.5kDa ATG4C protein using Rabbit Anti-ATG4C Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Human 293T (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG4C Polyclonal Antibody  at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~52.5kDa.
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Polyclonal Rabbit anti‑Human AUTL1 / ATG4C Antibody (APC, IF, WB) LS‑C775395

Polyclonal Rabbit anti‑Human AUTL1 / ATG4C Antibody (APC, IF, WB) LS‑C775395

Antibody:
AUTL1 / ATG4C Rabbit anti-Human Polyclonal (APC) Antibody
Application:
IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Allophycocyanin, Unmodified
Other formats:
Price
Catalog Number
$479
LS-C775395-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
AUTL1 / ATG4C Rabbit anti-Human Polyclonal (APC) Antibody
Application:
IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Allophycocyanin, Unmodified
Other formats:

Specifications

Description
ATG4C antibody LS-C775395 is an APC-conjugated rabbit polyclonal antibody to ATG4C (AUTL1) from human. It is reactive with human, mouse and rat. Validated for IF and WB.
Target
Human AUTL1 / ATG4C
Synonyms
ATG4C | AUTL3 | APG4-C | APG4C | AUTL1 | Autophagin-3 | APG4 autophagy 4 homolog C | Cysteine protease ATG4C
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Allophycocyanin. Also available conjugated with AP, Atto 390, Atto 488, Atto 594, Biotin, FITC, PerCP, RPE.
Purification
Peptide Affinity Purified
Modifications
Unmodified
Immunogen
Synthetic peptide from the N-Terminus of human ATG4C
Specificity
Predicted molecular weight at ~52.5 kDa. Observed molecular weights at ~45-60 kDa.
Applications
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
  • Applications tested for the base form of this product only
Usage
Applications should be user optimized.
Presentation
PBS, 0.09% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About AUTL1 / ATG4C
Q96DT6 NM_032852 NP_116241.2

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Requested From: United States
Date Requested: 4/24/2024