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Catalog Number Size Price
LS-C745289-25 25 µl (1 mg/ml) $275 
AURKB / Aurora-B Antibody - Western Blot shows detection of Aurora B protein at 39 kDa (predicted band size) in lane 1. Lane 1 : Extract from COS7 cells treated with Nocodazole (1ug/ml, 16 hrs). Lane 2 : Extract from COS7 cells treated with Nocodazole (1ug/ml, 16 hrs) incubated with the phosphopeptide immunogen. Primary Antibody : Aurora B (phospho T232) antibody diluted 1:500.

Polyclonal Rabbit anti‑Human AURKB / Aurora‑B Antibody (phospho‑Thr232, IF, WB) LS‑C745289

Polyclonal Rabbit anti‑Human AURKB / Aurora‑B Antibody (phospho‑Thr232, IF, WB) LS‑C745289

Antibody:
AURKB / Aurora-B Rabbit anti-Human Polyclonal (pThr232) Antibody
Application:
IF, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C745289-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
AURKB / Aurora-B Rabbit anti-Human Polyclonal (pThr232) Antibody
Application:
IF, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
Aurora-B antibody LS-C745289 is an unconjugated rabbit polyclonal antibody to Aurora-B (AURKB) (pThr232) from human. It is reactive with human and mouse. Validated for ELISA, IF and WB.
Target
Human AURKB / Aurora-B
Synonyms
AURKB | AIRK2 | AIK2 | Aurora kinase B | Aurora kinase B-Sv1 | Aurora-related kinase 2 | Aurora/IPL1-related kinase 2 | ARK-2 | ARK2 | Aurkb-sv1 | Aurkb-sv2 | Aurora 1 | Aurora-B | AIM-1 | IPL1 | STK-1 | STK5 | PPP1R48 | AurB | Aurora B | Aurora kinase B-Sv2 | Aurora-1 | Aurora1 | Serine/threonine kinase 12 | STK1 | STK12
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region surrounding T232 of Human Aurora Kinase B protein.
Epitope
pThr232
Specificity
Reactivity occurs against human Aurora Kinase B pT232 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Aurora Kinase B is minimal by ELISA. No reaction is expected against Aurora Kinase A. However, 100% sequence homology as indicated by BLAST analysis is on record for this protein from human, mouse, rat, cow, pig, dog and chimpanzee. Cross reactivity with Aurora Kinase B from other sources is not known.
Applications
  • Immunofluorescence
  • Western blot (1:250 - 1:2000)
  • ELISA (1:10000 - 1:50000)
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About AURKB / Aurora-B
Q96GD4 NM_004217 NP_004208.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/17/2021