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Catalog Number Size Price
LS-C204509-100 100 µl (1 mg/ml) $376 
AURKA / Aurora-A Antibody - Shows HeLa cell cultures stained with AURKA / Aurora-A antibody antibody (green). Aurora A localizes in spindle poles and mitotic spindles at anaphase and concentrates on the midbody between the two daughter cells during telophase. Counterstained is a chicken polyclonal antibody against Vimentin (red). Blue is a DNA stain.
AURKA / Aurora-A Antibody - Western analysis of AURKA / Aurora-A antibody. Blot of HeLa cells treated with 100 ng/mL of nocodazole for 18 hours was probed with AURKA / Aurora-A antibody. Nocodazole is a microtubule inhibitor which induces cells G2/M phase and induces Aurora A expression. The AURKA / Aurora-A antibody monoclonal binds strongly and cleanly to a band at about 46 kDa.
AURKA / Aurora-A Antibody - Shows HeLa cell cultures stained with AURKA / Aurora-A antibody antibody (green). Aurora A localizes in spindle poles and mitotic spindles at anaphase and concentrates on the midbody between the two daughter cells during telophase. Counterstained is a chicken polyclonal antibody against Vimentin (red). Blue is a DNA stain.
AURKA / Aurora-A Antibody - Western analysis of AURKA / Aurora-A antibody. Blot of HeLa cells treated with 100 ng/mL of nocodazole for 18 hours was probed with AURKA / Aurora-A antibody. Nocodazole is a microtubule inhibitor which induces cells G2/M phase and induces Aurora A expression. The AURKA / Aurora-A antibody monoclonal binds strongly and cleanly to a band at about 46 kDa.
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Monoclonal Mouse anti‑Human AURKA / Aurora‑A Antibody (clone 3H1, IF, WB) LS‑C204509

Monoclonal Mouse anti‑Human AURKA / Aurora‑A Antibody (clone 3H1, IF, WB) LS‑C204509

Antibody:
AURKA / Aurora-A Mouse anti-Human Monoclonal (3H1) Antibody
Application:
ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$376
LS-C204509-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
AURKA / Aurora-A Mouse anti-Human Monoclonal (3H1) Antibody
Application:
ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Aurora-A antibody LS-C204509 is an unconjugated mouse monoclonal antibody to human Aurora-A (AURKA). Validated for ICC, IF and WB.
Target
Human AURKA / Aurora-A
Synonyms
AURKA | AIK | AIRK1 | ARK1 | Aurora-A kinase | Aurora kinase A | Aurora/IPL1-like kinase | AYK1 | AURA | AURORA2 | Breast tumor-amplified kinase | Breast-tumor-amplified kinase | BTAK | HARK1 | IPL1-related kinase | Serine/threonine kinase 6 | STK7 | STK6 | PPP1R47 | ARK-1 | Aurora 2 | Aurora-A | Aurora-related kinase 1 | Aurora/IPL1-related kinase 1 | IAK1 | Serine/threonine kinase 15 | STK15
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
3H1
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Full length recombinant human Aurora A expressed in and purified from E. coli.
Specificity
Clone 3H1 recognizes Aurora A kinase specifically both in western blots and in immunocytochemical experiments. On blots, clone 3H1 reveals a prominent 46 kDa band, on cells in tissure cultures, clone 3H1 reveals strong staining in spindle poles and midbodies.
Applications
  • ICC (1:500 - 1:1000)
  • Immunofluorescence (1:500 - 1:1000)
  • Western blot (1:1000)
Usage
The antibody solution can be used at dilutions 1:500-1:1000 for immunofluorescence. For western blots try at 1:1000.
Presentation
PBS, 10 mM Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About AURKA / Aurora-A
O14965 NM_003600 NP_003591.2

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/25/2024