JOS antibody LS-B11516 is an unconjugated rabbit polyclonal antibody to JOS (ATXN3) from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Human, Monkey, Mouse, Rat, Bovine, Horse, Pig, Sheep
(tested or 100% immunogen sequence identity)
Also available conjugated with Biotin
- IHC - Paraffin (10 µg/ml)
- Western blot (0.2 - 1 µg/ml)
- (applications tested for the base form of this product only)
See our complete line of Immunohistochemistry Reagents
including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
ATXN3 / JOS antibody was raised against synthetic peptide located between aa35-84 of human ATXN3 (P54252, NP_004984). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Galago, Marmoset, Mouse, Rat, Sheep, Bovine, Horse, Pig, Platypus (100%); Xenopus, Stickleback, Zebrafish (92%); Monkey, Turkey, Zebra finch, Chicken (85%).
- Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B11516. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Human ATXN3 / Ataxin-3
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
For research use only.