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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ATXN3 / JOS Antibody (aa35‑84) IHC‑plus™ LS‑B11516
Note: This antibody replaces LS-C135245
JOS antibody LS-B11516 is an unconjugated rabbit polyclonal antibody to JOS (ATXN3) from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B11516-50
50 µg
$485
Description
JOS antibody LS-B11516 is an unconjugated rabbit polyclonal antibody to JOS (ATXN3) from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human ATXN3 / JOS
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Horse, Pig, Sheep (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, HRP, FITC.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
ATXN3 / JOS antibody was raised against synthetic peptide located between aa35-84 of human ATXN3 (P54252, NP_004984). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Galago, Marmoset, Mouse, Rat, Sheep, Bovine, Horse, Pig, Platypus (100%); Xenopus, Stickleback, Zebrafish (92%); Monkey, Turkey, Zebra finch, Chicken (85%).
Blocking Peptide
LS-E18231 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B11516. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa35-84
Specificity
Human ATXN3 / Ataxin-3
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About ATXN3 / JOS
P54252 NM_004993 NP_004984.2

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Images

Immunohistochemistry

Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

ATXN3 / Ataxin-3 antibody Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
ATXN3 / Ataxin-3 antibody Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

ATXN3 / Ataxin-3 antibody Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
ATXN3 / Ataxin-3 antibody Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

ATXN3 / Ataxin-3 antibody Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
ATXN3 / Ataxin-3 antibody Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

ATXN3 / Ataxin-3 antibody Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
ATXN3 / Ataxin-3 antibody Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 11/17/2018
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