Human ATXN1 / SCA1

Goat

Human, Monkey, Mouse, Rat, Bat, Bovine, Hamster, Horse, Pig (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Unmodified

Peptide with sequence C-TLKNLKNGSVKKGQ, from the internal region of the protein sequence according to NP_000323.2.

aa686-699

Human ATXN1 / Ataxin-1. Reported variants represent identical protein: NP_001121636.1, NP_000323.2

• Peptide Enzyme-Linked Immunosorbent Assay (1:32000)

Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Preliminary experiments gave bands at approx 75kD and 26kD in Mouse Fetal Brain lysates after 0.5 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 84.7kD according to NP_001186234.1 and 83.8kD according to NP_033150.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P54253 NCBI: NM_000332 NP_000323.2