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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C156660-400 400 µl $325 
ATP5J Antibody - ATP5J Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human liver tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
ATP5J Antibody - Fluorescent confocal image of U251 cell stained with ATP5J Antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ATP5J primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). ATP5J immunoreactivity is localized to Mitochondria significantly.
ATP5J Antibody - Western blot of lysate from rat heart tissue lysate, using ATP5J Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
ATP5J Antibody - ATP5J Antibody western blot of mouse stomach tissue lysates (35 ug/lane). The ATP5J antibody detected the ATP5J protein (arrow).
ATP5J Antibody - ATP5J Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human liver tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
ATP5J Antibody - Fluorescent confocal image of U251 cell stained with ATP5J Antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ATP5J primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). ATP5J immunoreactivity is localized to Mitochondria significantly.
ATP5J Antibody - Western blot of lysate from rat heart tissue lysate, using ATP5J Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
ATP5J Antibody - ATP5J Antibody western blot of mouse stomach tissue lysates (35 ug/lane). The ATP5J antibody detected the ATP5J protein (arrow).
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Polyclonal Rabbit anti‑Human ATP5J Antibody (aa28‑56, IHC, IF, WB) LS‑C156660

Polyclonal Rabbit anti‑Human ATP5J Antibody (aa28‑56, IHC, IF, WB) LS‑C156660

Antibody:
Rabbit Polyclonal to Human ATP5J
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$325 
LS-C156660-400
400 µl
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human ATP5J
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
ATP5J antibody LS-C156660 is an unconjugated rabbit polyclonal antibody to ATP5J (aa28-56) from human. It is reactive with human and mouse. Validated for IF, IHC and WB.
Target
Human ATP5J
Synonyms
ATP5J | ATP5 | ATP5A | Coupling factor 6 | CF6 | F6 | ATPase subunit F6 | ATPM | ATP5PF | ATP synthase-coupling factor 6, mitochondrial | P18859 | ATP5J_HUMAN
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Predicted
Monkey (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:10 - 1:50)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E1689 - Lyophilized - 500 µg - $360.00
Immunizing peptide used to generate LS-C156660. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa28-56
Specificity
This ATP5J antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 28-56 amino acids from the Central region of human ATP5J.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ATP5J

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Requested From: United States
Date Requested: 6/5/2020