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Catalog Number Size Price
LS-C776037-100 100 µg (1 mg/ml) $477 
ATG3 Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG3 Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG3 Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ATG3 Antibody. (D) Composite.
ATG3 Antibody - Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~35.9 kDa ATG3 protein using Rabbit Anti-ATG3 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG3 Polyclonal Antibody  at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~35.9 kDa.
ATG3 Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG3 Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG3 Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ATG3 Antibody. (D) Composite.
ATG3 Antibody - Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~35.9 kDa ATG3 protein using Rabbit Anti-ATG3 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG3 Polyclonal Antibody  at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~35.9 kDa.
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Polyclonal Rabbit anti‑Human ATG3 Antibody (RPE, IF, WB) LS‑C776037

Polyclonal Rabbit anti‑Human ATG3 Antibody (RPE, IF, WB) LS‑C776037

Antibody:
ATG3 Rabbit anti-Human Polyclonal (RPE) Antibody
Application:
IF, WB
Reactivity:
Human
Format:
R. Phycoerythrin, Unmodified
Other formats:
Price
Catalog Number
$477
LS-C776037-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
ATG3 Rabbit anti-Human Polyclonal (RPE) Antibody
Application:
IF, WB
Reactivity:
Human
Format:
R. Phycoerythrin, Unmodified
Other formats:

Specifications

Description
ATG3 antibody LS-C776037 is an RPE-conjugated rabbit polyclonal antibody to human ATG3. Validated for IF and WB.
Target
Human ATG3
Synonyms
ATG3 | APG3-LIKE | APG3 | APG3L | Autophagy related 3 | HApg3 | PC3-96 | Protein PC3-96 | Autophagy-related protein 3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
R. Phycoerythrin. Also available conjugated with AP, APC, Atto 390, Atto 488, Atto 594, Biotin, FITC, PerCP.
Purification
Peptide Affinity Purified
Modifications
Unmodified
Immunogen
Synthetic peptide from the mid-protein of Human ATG3
Specificity
Detects ~35 kDa.
Applications
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
  • Applications tested for the base form of this product only
Usage
Applications should be user optimized.
Presentation
PBS, 0.09% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ATG3
Q9NT62 NM_022488 NP_071933.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/16/2024