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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ATF6 Antibody (N‑Terminus) IHC‑plus™ LS‑B2430
Note: This antibody replaces LS-C19566
ATF6 antibody LS-B2430 is an unconjugated rabbit polyclonal antibody to ATF6 from human and mouse. Validated for ELISA, ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B2430-50
50 µg (1 mg/ml)
$425

Popular ATF6 Products

Anti-ATF6 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2564 concentration 10 ug/ml.
Species: Human, Mouse, Rat, Hamster
Applications: IHC, IHC - Paraffin, ICC, Western blot, Chromatin Immunoprecipitation
Anti-ATF6 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2516 concentration 5 ug/ml.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Anti-ATF6 antibody IHC of mouse adipose. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5587 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse, Human, Monkey, Rat, Bovine, Guinea pig, Horse, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Western blot
Anti-ATF6 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6032 concentration 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA

Product Description

LifeSpan Antibody

Specifications

Target
Human ATF6
Synonyms
ATF6, ATF6-alpha, ATF6A
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • ICC
  • Immunofluorescence
  • Western blot (0.5 - 1 µg/ml)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E30207 - Liquid - 50 µg 0.2 mg/ml - $145.00
Immunizing peptide used to generate LS-B2430. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
N-Terminus
Usage
Immunohistochemistry: LS-B2430 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2430 was determined to be 5 ug/ml.
Presentation
PBS, 0.02% Sodium Azide
Storage
Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only.
About ATF6
ATF6 is a transcription factor that activates target genes for the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Although it is a transcription factor, this protein is unusual in that it is synthesized as a transmembrane protein that is embedded in the ER. P18850 NM_007348 NP_031374.2


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Images

Immunohistochemistry

Anti-ATF6 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2430 concentration 5 ug/ml.
Anti-ATF6 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2430 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of ATF6 in A20 with ATF6 antibody at 10 ug/ml.
Immunocytochemistry of ATF6 in A20 with ATF6 antibody at 10 ug/ml.

Western blot

Western blot of ATF6 in MDA-MB-361 cell lysate with ATF6 antibody at (A) 0.5 and (B) 1 ug/ml.
Western blot of ATF6 in MDA-MB-361 cell lysate with ATF6 antibody at (A) 0.5 and (B) 1 ug/ml.

Immunofluorescence

Immunofluorescence of ATF6 in MCF7 cells with ATF6 Antibody at 20 µg/mL.
Immunofluorescence of ATF6 in MCF7 cells with ATF6 Antibody at 20 µg/mL.

Western blot

Western blot analysis of ATF6 in MCF7 cell lysate with ATF6 antibody at (A) 1 and (B) 2 µg/mL.
Western blot analysis of ATF6 in MCF7 cell lysate with ATF6 antibody at (A) 1 and (B) 2 µg/mL.

Immunohistochemistry

Anti-ATF6 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2430 concentration 5 ug/ml.
Anti-ATF6 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2430 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of ATF6 in A20 with ATF6 antibody at 10 ug/ml.
Immunocytochemistry of ATF6 in A20 with ATF6 antibody at 10 ug/ml.

Western blot

Western blot of ATF6 in MDA-MB-361 cell lysate with ATF6 antibody at (A) 0.5 and (B) 1 ug/ml.
Western blot of ATF6 in MDA-MB-361 cell lysate with ATF6 antibody at (A) 0.5 and (B) 1 ug/ml.

Immunofluorescence

Immunofluorescence of ATF6 in MCF7 cells with ATF6 Antibody at 20 µg/mL.
Immunofluorescence of ATF6 in MCF7 cells with ATF6 Antibody at 20 µg/mL.

Western blot

Western blot analysis of ATF6 in MCF7 cell lysate with ATF6 antibody at (A) 1 and (B) 2 µg/mL.
Western blot analysis of ATF6 in MCF7 cell lysate with ATF6 antibody at (A) 1 and (B) 2 µg/mL.

Immunohistochemistry

Anti-ATF6 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2430 concentration 5 ug/ml.
Anti-ATF6 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2430 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of ATF6 in A20 with ATF6 antibody at 10 ug/ml.
Immunocytochemistry of ATF6 in A20 with ATF6 antibody at 10 ug/ml.

Western blot

Western blot of ATF6 in MDA-MB-361 cell lysate with ATF6 antibody at (A) 0.5 and (B) 1 ug/ml.
Western blot of ATF6 in MDA-MB-361 cell lysate with ATF6 antibody at (A) 0.5 and (B) 1 ug/ml.

Immunofluorescence

Immunofluorescence of ATF6 in MCF7 cells with ATF6 Antibody at 20 µg/mL.
Immunofluorescence of ATF6 in MCF7 cells with ATF6 Antibody at 20 µg/mL.

Western blot

Western blot analysis of ATF6 in MCF7 cell lysate with ATF6 antibody at (A) 1 and (B) 2 µg/mL.
Western blot analysis of ATF6 in MCF7 cell lysate with ATF6 antibody at (A) 1 and (B) 2 µg/mL.

Immunohistochemistry

Anti-ATF6 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2430 concentration 5 ug/ml.
Anti-ATF6 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2430 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of ATF6 in A20 with ATF6 antibody at 10 ug/ml.
Immunocytochemistry of ATF6 in A20 with ATF6 antibody at 10 ug/ml.

Western blot

Western blot of ATF6 in MDA-MB-361 cell lysate with ATF6 antibody at (A) 0.5 and (B) 1 ug/ml.
Western blot of ATF6 in MDA-MB-361 cell lysate with ATF6 antibody at (A) 0.5 and (B) 1 ug/ml.

Immunofluorescence

Immunofluorescence of ATF6 in MCF7 cells with ATF6 Antibody at 20 µg/mL.
Immunofluorescence of ATF6 in MCF7 cells with ATF6 Antibody at 20 µg/mL.

Western blot

Western blot analysis of ATF6 in MCF7 cell lysate with ATF6 antibody at (A) 1 and (B) 2 µg/mL.
Western blot analysis of ATF6 in MCF7 cell lysate with ATF6 antibody at (A) 1 and (B) 2 µg/mL.

Requested From: United States
Date Requested: 10/15/2018

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