ATF6 antibody LS-B2388 is an unconjugated goat polyclonal antibody to ATF6 from human and rabbit. Validated for IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues.
(tested or 100% immunogen sequence identity)
Mouse, Bovine, Hamster, Horse, Pig (at least 90% immunogen sequence identity)
- IHC - Paraffin (2.5 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:8000)
See our complete line of Immunohistochemistry Reagents
including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
ATF6 antibody was raised against synthetic peptide (C-EATHVVSTIPESLQ) from C-terminus of human ATF6. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Elephant, Rabbit (100%); Mouse, Hamster, Bovine, Horse, Pig (93%); Monkey, Panda (86%).
Immunohistochemistry: LS-B2388 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2388 was determined to be 2.5 ug/ml.
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.