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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ATF6 Antibody (C‑Terminus) IHC‑plus™ LS‑B2516
Note: This antibody replaces LS-C19565
ATF6 antibody LS-B2516 is an unconjugated rabbit polyclonal antibody to ATF6 from human and mouse. Validated for ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B2516-50
50 µg (1 mg/ml)
$425

Popular ATF6 Products

Anti-ATF6 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2564 concentration 10 ug/ml.
Species: Human, Mouse, Rat, Hamster
Applications: IHC, IHC - Paraffin, ICC, Western blot, Chromatin Immunoprecipitation
Anti-ATF6 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2430 concentration 5 ug/ml.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Anti-ATF6 antibody IHC of mouse adipose. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5587 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse, Human, Monkey, Rat, Bovine, Guinea pig, Horse, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Western blot
Anti-ATF6 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6032 concentration 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA

Product Description

ATF6 antibody LS-B2516 is an unconjugated rabbit polyclonal antibody to ATF6 from human and mouse. Validated for ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About ATF6
ATF6 is a transcription factor that activates target genes for the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Although it is a transcription factor, this protein is unusual in that it is synthesized as a transmembrane protein that is embedded in the ER. P18850 NM_007348 NP_031374.2

ATF6 Antibody, ATF6-alpha Antibody, ATF6A Antibody


Specifications

Target
Human ATF6
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • ICC
  • Immunofluorescence
  • Western blot (0.5 - 1 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E30206 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-B2516. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Usage
Immunohistochemistry: LS-B2516 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2516 was determined to be 5 ug/ml.
Presentation
PBS, 0.02% Sodium Azide
Storage
Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only.

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Images

Immunohistochemistry

Anti-ATF6 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2516 concentration 5 ug/ml.
Anti-ATF6 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2516 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemical staining of MCF7 cells using ATF6 antibody at 10 ug/ml.
Immunocytochemical staining of MCF7 cells using ATF6 antibody at 10 ug/ml.

Immunofluorescence

Immunofluorescence of ATF6 in human breast tissue with ATF6 antibody at 20 µg/ml.Green: ATF6 Antibody  Blue: DAPI staining
Immunofluorescence of ATF6 in human breast tissue with ATF6 antibody at 20 µg/ml.Green: ATF6 Antibody Blue: DAPI staining

Immunofluorescence

Immunofluorescence of ATF6 in human pancreas tissue with ATF6 antibody at 20 µg/ml.Green: ATF6 Antibody  Blue: DAPI staining
Immunofluorescence of ATF6 in human pancreas tissue with ATF6 antibody at 20 µg/ml.Green: ATF6 Antibody Blue: DAPI staining

Immunofluorescence

Immunofluorescence of ATF6 in MCF7 cells with ATF6 antibody at 10 ug/ml.
Immunofluorescence of ATF6 in MCF7 cells with ATF6 antibody at 10 ug/ml.

Immunohistochemistry

Anti-ATF6 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2516 concentration 5 ug/ml.
Anti-ATF6 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2516 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemical staining of MCF7 cells using ATF6 antibody at 10 ug/ml.
Immunocytochemical staining of MCF7 cells using ATF6 antibody at 10 ug/ml.

Immunofluorescence

Immunofluorescence of ATF6 in human breast tissue with ATF6 antibody at 20 µg/ml.Green: ATF6 Antibody  Blue: DAPI staining
Immunofluorescence of ATF6 in human breast tissue with ATF6 antibody at 20 µg/ml.Green: ATF6 Antibody Blue: DAPI staining

Immunofluorescence

Immunofluorescence of ATF6 in human pancreas tissue with ATF6 antibody at 20 µg/ml.Green: ATF6 Antibody  Blue: DAPI staining
Immunofluorescence of ATF6 in human pancreas tissue with ATF6 antibody at 20 µg/ml.Green: ATF6 Antibody Blue: DAPI staining

Immunofluorescence

Immunofluorescence of ATF6 in MCF7 cells with ATF6 antibody at 10 ug/ml.
Immunofluorescence of ATF6 in MCF7 cells with ATF6 antibody at 10 ug/ml.

Immunohistochemistry

Anti-ATF6 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2516 concentration 5 ug/ml.
Anti-ATF6 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2516 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemical staining of MCF7 cells using ATF6 antibody at 10 ug/ml.
Immunocytochemical staining of MCF7 cells using ATF6 antibody at 10 ug/ml.

Immunofluorescence

Immunofluorescence of ATF6 in human breast tissue with ATF6 antibody at 20 µg/ml.Green: ATF6 Antibody  Blue: DAPI staining
Immunofluorescence of ATF6 in human breast tissue with ATF6 antibody at 20 µg/ml.Green: ATF6 Antibody Blue: DAPI staining

Immunofluorescence

Immunofluorescence of ATF6 in human pancreas tissue with ATF6 antibody at 20 µg/ml.Green: ATF6 Antibody  Blue: DAPI staining
Immunofluorescence of ATF6 in human pancreas tissue with ATF6 antibody at 20 µg/ml.Green: ATF6 Antibody Blue: DAPI staining

Immunofluorescence

Immunofluorescence of ATF6 in MCF7 cells with ATF6 antibody at 10 ug/ml.
Immunofluorescence of ATF6 in MCF7 cells with ATF6 antibody at 10 ug/ml.

Immunohistochemistry

Anti-ATF6 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2516 concentration 5 ug/ml.
Anti-ATF6 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2516 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemical staining of MCF7 cells using ATF6 antibody at 10 ug/ml.
Immunocytochemical staining of MCF7 cells using ATF6 antibody at 10 ug/ml.

Immunofluorescence

Immunofluorescence of ATF6 in human breast tissue with ATF6 antibody at 20 µg/ml.Green: ATF6 Antibody  Blue: DAPI staining
Immunofluorescence of ATF6 in human breast tissue with ATF6 antibody at 20 µg/ml.Green: ATF6 Antibody Blue: DAPI staining

Immunofluorescence

Immunofluorescence of ATF6 in human pancreas tissue with ATF6 antibody at 20 µg/ml.Green: ATF6 Antibody  Blue: DAPI staining
Immunofluorescence of ATF6 in human pancreas tissue with ATF6 antibody at 20 µg/ml.Green: ATF6 Antibody Blue: DAPI staining

Immunofluorescence

Immunofluorescence of ATF6 in MCF7 cells with ATF6 antibody at 10 ug/ml.
Immunofluorescence of ATF6 in MCF7 cells with ATF6 antibody at 10 ug/ml.

Requested From: United States
Date Requested: 9/22/2018

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