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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ATF2 Antibody (phospho‑Ser90) LS‑C3472
ATF2 antibody LS-C3472 is an unconjugated sheep polyclonal antibody to human ATF2. Validated for Func and WB.
Antibody
Catalog
Size
Price
LS-C3472-200
200 µg
$575

Popular ATF2 Products

Anti-ATF2 antibody IHC of human small intestine, submucosal plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6524 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
IHC of paraffin-embedded lung cancer (left) and brain tissues (right) using ATF2 mouse monoclonal antibody with DAB staining.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
ATF2 antibody LS-C31200 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Hamster, Horse, Rabbit, Chicken
Applications: IHC, IHC - Paraffin, Western blot
Staining (0.5?g/ml) of Human Placenta lysate (RIPA buffer, 35?g total protein per lane). Detected by chemiluminescence.
Species: Human, Monkey, Bovine, Pig, Rabbit
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Anti-ATF2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1170 concentration 10 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot

Product Description

ATF2 antibody LS-C3472 is an unconjugated sheep polyclonal antibody to human ATF2. Validated for Func and WB.

Specifications

Target
Human ATF2
Synonyms
ATF2, CREB-2, CRE-BP1, CREB2, HB16, TREB7, CREBP1
Host
Sheep
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • Western blot (0.5 - 2 µg/ml)
  • Functional Assay
  • (applications tested for the base form of this product only)
Immunogen
ATF2 antibody was raised against kLH-conjugated, synthetic peptide corresponding to amino acids surrounding phosphorylated Ser90 of human ATF2. The immunizing sequence is identical in human, mouse and rat.
Epitope
pSer90
Specificity
Recognizes ATF2 phosphorylated by JNK1a1/SAPK1c, active. Species cross-reactivity: Human. Predicted to cross-react with mouse and rat, based on sequence homology.
Usage
Suitable for use in Western Blot. Western Blot: 0.5-2.0 ug/ml Detects recombinant ATF2, phosphorylated by JNK1 1/SAPK1c, active, using a cold kinase assay. ATF2 and ATF2 phosphorylated by JNK1 1/SAPK1c, active were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-ATF2 (Ser90) (0.5 ug/ml). Proteins were visualized using a rabbit anti-sheep secondary antibody conjugated to HRP and a chemiluminescence detection system.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About ATF2
Transcriptional activator which regulates the transcription of various genes, including those involved in anti-apoptosis, cell growth, and DNA damage response. Dependent on its binding partner, binds to CRE (cAMP response element) consensus sequences (5'-TGACGTCA-3') or to AP-1 (activator protein 1) consensus sequences (5'-TGACTCA-3'). P15336 NM_001880 NP_001871.2


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Requested From: United States
Date Requested: 10/19/2018

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