ATF2 antibody LS-C3472 is an unconjugated sheep polyclonal antibody to human ATF2. Validated for Func and WB.
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein G purified
- Western blot (0.5 - 2 µg/ml)
- Functional Assay
- (applications tested for the base form of this product only)
ATF2 antibody was raised against kLH-conjugated, synthetic peptide corresponding to amino acids surrounding phosphorylated Ser90 of human ATF2. The immunizing sequence is identical in human, mouse and rat.
Recognizes ATF2 phosphorylated by JNK1a1/SAPK1c, active. Species cross-reactivity: Human. Predicted to cross-react with mouse and rat, based on sequence homology.
Suitable for use in Western Blot. Western Blot: 0.5-2.0 ug/ml Detects recombinant ATF2, phosphorylated by JNK1 1/SAPK1c, active, using a cold kinase assay. ATF2 and ATF2 phosphorylated by JNK1 1/SAPK1c, active were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-ATF2 (Ser90) (0.5 ug/ml). Proteins were visualized using a rabbit anti-sheep secondary antibody conjugated to HRP and a chemiluminescence detection system.
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.