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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ASAP1 Antibody (aa777-787) LS-C19043

ASAP1 antibody LS-C19043 is an unconjugated rabbit polyclonal antibody to ASAP1 from human, mouse, rat and other species. Validated for ELISA, ICC, IF and WB.
Catalog
Size
Price
LS-C19043-100
100 µg (0.95 mg/ml)
$425
Description
ASAP1 antibody LS-C19043 is an unconjugated rabbit polyclonal antibody to ASAP1 from human, mouse, rat and other species. Validated for ELISA, ICC, IF and WB.
Target
Mouse ASAP1
Host
Rabbit
Reactivity
Mouse, Human, Rat, Bovine, Dog, Chicken (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • ICC
  • Immunofluorescence
  • Western blot (1:500 - 1:2000)
  • ELISA (1:2000 - 1:10000)
Immunogen
ASAP1 antibody was raised against synthetic peptide from mouse ASAP1.
Epitope
aa777-787
Specificity
Amino acids 777-787 of mouse ASAP1 protein (see below).
Usage
This affinity purified antibody has been tested for use in ELISA, IF microscopy and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 130 kD in size corresponding to ASAP1 protein by western blotting in the appropriate cell lysate or extract. This antibody recognizes both phosphorylated and nonphosphorylated ASAP1 at amino acid Y782.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About ASAP1
Q9ULH1 NM_018482 NP_060952.2

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Western Blot of rabbit anti-ASAP1 antibody. Lane 1: ASAP1 transfected lysate. Load: 35 µg per lane. Primary antibody: ASAP1 antibody at 1:1000 for overnight at 4°C. Secondary antibody: rabbit secondary HRP antibody at 1:70,000 for 30 min at RT. Block: BlockOut overnight at 4°C. Predicted/Observed size: 125 kda for ASAP1.
Species: Human
Applications: Western blot, ELISA

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Immunofluorescence

Anti-ASAP1 Antibody - Immunofluorescent Microscopy. Immunofluorescent microscopy using Affinity Purified anti-ASAP1 antibody shows detection of ASAP1present in mouse NIH3T3 cells transfected with activated Src. Specific staining is not present when antibody is pre-incubated with the immunizing peptide prior to reaction with cells. Personal Communication. Paul Randazzo, NIH, CCR, Bethesda, MD.
Anti-ASAP1 Antibody - Immunofluorescent Microscopy. Immunofluorescent microscopy using Affinity Purified anti-ASAP1 antibody shows detection of ASAP1present in mouse NIH3T3 cells transfected with activated Src. Specific staining is not present when antibody is pre-incubated with the immunizing peptide prior to reaction with cells. Personal Communication. Paul Randazzo, NIH, CCR, Bethesda, MD.

Immunofluorescence

Anti-ASAP1 Antibody - Immunofluorescent Microscopy. Immunofluorescent microscopy using Affinity Purified anti-ASAP1 antibody shows detection of ASAP1present in mouse NIH3T3 cells transfected with activated Src. Specific staining is not present when antibody is pre-incubated with the immunizing peptide prior to reaction with cells. Personal Communication. Paul Randazzo, NIH, CCR, Bethesda, MD.
Anti-ASAP1 Antibody - Immunofluorescent Microscopy. Immunofluorescent microscopy using Affinity Purified anti-ASAP1 antibody shows detection of ASAP1present in mouse NIH3T3 cells transfected with activated Src. Specific staining is not present when antibody is pre-incubated with the immunizing peptide prior to reaction with cells. Personal Communication. Paul Randazzo, NIH, CCR, Bethesda, MD.

Immunofluorescence

Anti-ASAP1 Antibody - Immunofluorescent Microscopy. Immunofluorescent microscopy using Affinity Purified anti-ASAP1 antibody shows detection of ASAP1present in mouse NIH3T3 cells transfected with activated Src. Specific staining is not present when antibody is pre-incubated with the immunizing peptide prior to reaction with cells. Personal Communication. Paul Randazzo, NIH, CCR, Bethesda, MD.
Anti-ASAP1 Antibody - Immunofluorescent Microscopy. Immunofluorescent microscopy using Affinity Purified anti-ASAP1 antibody shows detection of ASAP1present in mouse NIH3T3 cells transfected with activated Src. Specific staining is not present when antibody is pre-incubated with the immunizing peptide prior to reaction with cells. Personal Communication. Paul Randazzo, NIH, CCR, Bethesda, MD.

Immunofluorescence

Anti-ASAP1 Antibody - Immunofluorescent Microscopy. Immunofluorescent microscopy using Affinity Purified anti-ASAP1 antibody shows detection of ASAP1present in mouse NIH3T3 cells transfected with activated Src. Specific staining is not present when antibody is pre-incubated with the immunizing peptide prior to reaction with cells. Personal Communication. Paul Randazzo, NIH, CCR, Bethesda, MD.
Anti-ASAP1 Antibody - Immunofluorescent Microscopy. Immunofluorescent microscopy using Affinity Purified anti-ASAP1 antibody shows detection of ASAP1present in mouse NIH3T3 cells transfected with activated Src. Specific staining is not present when antibody is pre-incubated with the immunizing peptide prior to reaction with cells. Personal Communication. Paul Randazzo, NIH, CCR, Bethesda, MD.

Requested From: United States
Date Requested: 4/21/2019
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