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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
Infectious Disease
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Catalog Number Size Price
LS-C19043-100 100 µg (0.95 mg/ml) $425 
ASAP1 Antibody - Anti-ASAP1 Antibody - Immunofluorescent Microscopy. Immunofluorescent microscopy using Affinity Purified anti-ASAP1 antibody shows detection of ASAP1present in mouse NIH3T3 cells transfected with activated Src. Specific staining is not present when antibody is pre-incubated with the immunizing peptide prior to reaction with cells. Personal Communication. Paul Randazzo, NIH, CCR, Bethesda, MD.

Polyclonal ASAP1 Antibody (IF, WB) LS‑C19043

Polyclonal ASAP1 Antibody (IF, WB) LS‑C19043

Antibody:
Rabbit Polyclonal to Mouse ASAP1
Application:
IF, WB, ELISA
Reactivity:
Mouse, Human, Chimpanzee, Rat, Bovine, Dog, Chicken
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C19043-100
100 µg
0.95 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Mouse ASAP1
Application:
IF, WB, ELISA
Reactivity:
Mouse, Human, Chimpanzee, Rat, Bovine, Dog, Chicken
Format:
Unconjugated, Unmodified

Specifications

Description
ASAP1 antibody LS-C19043 is an unconjugated rabbit polyclonal antibody to ASAP1 from human, mouse, rat and other species. Validated for ELISA, IF and WB.
Target
Mouse ASAP1
Synonyms
ASAP1 | AMAP1 | Centaurin, beta 4 | CENTB4 | DEF-1 | DDEF1 | KIAA1249 | PAP | PIP2-dependent ARF1 GAP | PAG2 | ZG14P
Host
Rabbit
Reactivity
Mouse, Human, Chimpanzee, Rat, Bovine, Dog, Chicken (tested or 100% immunogen sequence identity)
Predicted
Human, Chimpanzee, Rat, Bovine, Dog, Chicken (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • Immunofluorescence
  • Western blot (1:500 - 1:2000)
  • ELISA (1:2000 - 1:10000)
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 775-800 of mouse ASAP1 protein.
Specificity
A BLAST analysis was used to suggest cross reactivity with ASAP1 proteins from human, chicken, bovine, dog, rat and chimpanzee based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Usage
This affinity purified antibody has been tested for use in ELISA, IF microscopy and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 130 kD in size corresponding to ASAP1 protein by western blotting in the appropriate cell lysate or extract. This antibody recognizes both phosphorylated and nonphosphorylated ASAP1 at amino acid Y782.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ASAP1
Q9ULH1 NM_018482 NP_060952.2

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Requested From: United States
Date Requested: 2/22/2020