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Catalog Number Size Price
LS-C748963-20 20 µl $253 
LS-C748963-50 50 µl $279 
LS-C748963-100 100 µl $333 
LS-C748963-200 200 µl $429 
ASAH1 / Acid Ceramidase Antibody - Western blot analysis of extracts of various cell lines, using ASAH1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.

Polyclonal Rabbit anti‑Human ASAH1 / Acid Ceramidase Antibody (WB) LS‑C748963

Polyclonal Rabbit anti‑Human ASAH1 / Acid Ceramidase Antibody (WB) LS‑C748963

Antibody:
ASAH1 / Acid Ceramidase Rabbit anti-Human Polyclonal Antibody
Application:
WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$253
LS-C748963-20
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
ASAH1 / Acid Ceramidase Rabbit anti-Human Polyclonal Antibody
Application:
WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Acid Ceramidase antibody LS-C748963 is an unconjugated rabbit polyclonal antibody to Acid Ceramidase (ASAH1) from human. It is reactive with human, mouse and rat. Validated for WB.
Target
Human ASAH1 / Acid Ceramidase
Synonyms
ASAH1 | ACDase | Acid CDase | Acid ceramidase | ASAH | Acylsphingosine deacylase | PHP32 | SMAPME | PHP | Putative 32 kDa heart protein
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 140-389 of human ASAH1 (NP_001120977.1). IVAEDKKGHLIHGRNMDFGVFLGWNINNDTWVITEQLKPLTVNLDFQRNNKTVFKASSFAGYVGMLTGFKPGLFSLTLNERFSINGGYLGILEWILGKKDVMWIGFLTRTVLENSTSYEEAKNLLTKTKILAPAYFILGGNQSGEGCVITRDRKESLDVYELDAKQGRWYVVQTNYDRWKHPFFLDDRRTPAKMCLNRTSQENISFETMYDVLSTKPVLNKLTVYTTLIDVTKGQFETYLRDCPDPCIGW
Applications
  • Western blot (1:500 - 1:2000)
Usage
The predicted MW is 44kDa/46kDa, while the observed MW by Western blot was 46kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ASAH1 / Acid Ceramidase
Q13510 NM_004315 NP_004306.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 1/22/2022