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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C498-100 100 µg (0.5 mg/ml) $335 
AR / Androgen Receptor Antibody - LNCaP cells (passage number 38) were serum-starved for 2 days. After serum starvation, cells were (A)left untreated, (B) treated with 100 ng/ml IGF-1 for 4h, or (C) incubated with 20 um LY294002 for 30 min prior to treatment with 100 ng/ml IGF-1 for 4 h.

Monoclonal Mouse anti‑Human AR / Androgen Receptor Antibody (clone 156C135.2, phospho‑Ser213, WB) LS‑C498

Monoclonal Mouse anti‑Human AR / Androgen Receptor Antibody (clone 156C135.2, phospho‑Ser213, WB) LS‑C498

Note: This antibody replaces LS-C69003, LS-C20617
Antibody:
AR / Androgen Receptor Mouse anti-Human Monoclonal (pSer213) (156C135.2) Antibody
Application:
WB
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Price
Catalog Number
$335
LS-C498-100
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
AR / Androgen Receptor Mouse anti-Human Monoclonal (pSer213) (156C135.2) Antibody
Application:
WB
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified

Specifications

Description
Androgen Receptor antibody LS-C498 is an unconjugated mouse monoclonal antibody to Androgen Receptor (AR) (pSer213) from human. It is reactive with human and monkey. Validated for WB.
Target
Human AR / Androgen Receptor
Synonyms
AR | Androgen receptor | Dihydrotestosterone receptor | DHTR | AIS | KD | NR3C4 | HUMARA | SBMA | TFM | SMAX1 | HYSP1
Host
Mouse
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Predicted
Bat (at least 90% immunogen sequence identity)
Clonality
IgG Monoclonal
Clone
156C135.2
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
This antibody was developed against a synthetic peptide corresponding to amino acids 207-221 (GRAREAS*GAPTSSKD) of human androgen receptor, containing the serine 213 phosphorylation site: GenBank Accession No. A39248. Note: S* refers to phosphorylated serine in the peptide sequence. Percent identity by BLAST analysis: Human, Chimpanzee, Baboon, Monkey, Marmoset (100%); Gorilla, Bat, Panda (93%); Dog, Bovine, Cat, Rabbit, Horse, Pig, Guinea pig (87%); Mouse, Rat, Hamster (80%).
Epitope
pSer213
Specificity
In IGF-1 stimulated LNCaP cells (passage number 38), a ~110 kD band was observed. Please see Lin et al. 2003 for additional details. The serine phosphorylation site recognized by this has been alternatively referred to Ser213 (Lee and Chang, 2003) and Ser210 (Lin et al, 2003). Variations in denotation can arise from how the sequence is counted in various GenBank accession numbers. The site is denoted as Ser213 in GenBank Accession No. A39248, which was used to design the immunogen.
Applications
  • Western blot (1 - 4 µg/ml)
Presentation
PBS, 0.05% Sodium Azide, 0.2% Gelatin
Storage
Short term: 4°C; Long term: -70°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About AR / Androgen Receptor
P10275 NM_000044 NP_000035.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/26/2020