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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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AR / Androgen Receptor Antibody (phospho‑Ser213, clone 156C135.2) LS‑C498
Note: This antibody replaces LS-C69003, LS-C20617
Androgen Receptor antibody LS-C498 is an unconjugated mouse monoclonal antibody to Androgen Receptor (AR) from human and monkey. Validated for WB.
Catalog
Size
Price
LS-C498-100
100 µg (0.5 mg/ml)
$335
Description
Androgen Receptor antibody LS-C498 is an unconjugated mouse monoclonal antibody to Androgen Receptor (AR) from human and monkey. Validated for WB.
Target
Human AR / Androgen Receptor
Host
Mouse
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Predicted
Bat (at least 90% immunogen sequence identity)
Clonality
IgG Monoclonal [156C135.2]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • Western blot (1 - 4 µg/ml)
Immunogen
This antibody was developed against a synthetic peptide corresponding to amino acids 207-221 (GRAREAS*GAPTSSKD) of human androgen receptor, containing the serine 213 phosphorylation site: GenBank Accession No. A39248. Note: S* refers to phosphorylated serine in the peptide sequence. Percent identity by BLAST analysis: Human, Chimpanzee, Baboon, Monkey, Marmoset (100%); Gorilla, Bat, Panda (93%); Dog, Bovine, Cat, Rabbit, Horse, Pig, Guinea pig (87%); Mouse, Rat, Hamster (80%).
Epitope
pSer213
Specificity
In IGF-1 stimulated LNCaP cells (passage number 38), a ~110 kD band was observed. Please see Lin et al. 2003 for additional details. The serine phosphorylation site recognized by this has been alternatively referred to Ser213 (Lee and Chang, 2003) and Ser210 (Lin et al, 2003). Variations in denotation can arise from how the sequence is counted in various GenBank accession numbers. The site is denoted as Ser213 in GenBank Accession No. A39248, which was used to design the immunogen.
Presentation
PBS, 0.05% Sodium Azide, 0.2% Gelatin
Storage
Short term: 4°C; Long term: -70°C.
Restrictions
For research use only.
About AR / Androgen Receptor
P10275 NM_000044 NP_000035.2

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Images

Western blot

LNCaP cells (passage number 38) were serum-starved for 2 days. After serum starvation, cells were (A)left untreated, (B) treated with 100 ng/ml IGF-1 for 4h, or (C) incubated with 20 um LY294002 for 30 min prior to treatment with 100 ng/ml IGF-1 for 4 h.
LNCaP cells (passage number 38) were serum-starved for 2 days. After serum starvation, cells were (A)left untreated, (B) treated with 100 ng/ml IGF-1 for 4h, or (C) incubated with 20 um LY294002 for 30 min prior to treatment with 100 ng/ml IGF-1 for 4 h.

Western blot

LNCaP cells (passage number 38) were serum-starved for 2 days. After serum starvation, cells were (A)left untreated, (B) treated with 100 ng/ml IGF-1 for 4h, or (C) incubated with 20 um LY294002 for 30 min prior to treatment with 100 ng/ml IGF-1 for 4 h.
LNCaP cells (passage number 38) were serum-starved for 2 days. After serum starvation, cells were (A)left untreated, (B) treated with 100 ng/ml IGF-1 for 4h, or (C) incubated with 20 um LY294002 for 30 min prior to treatment with 100 ng/ml IGF-1 for 4 h.

Western blot

LNCaP cells (passage number 38) were serum-starved for 2 days. After serum starvation, cells were (A)left untreated, (B) treated with 100 ng/ml IGF-1 for 4h, or (C) incubated with 20 um LY294002 for 30 min prior to treatment with 100 ng/ml IGF-1 for 4 h.
LNCaP cells (passage number 38) were serum-starved for 2 days. After serum starvation, cells were (A)left untreated, (B) treated with 100 ng/ml IGF-1 for 4h, or (C) incubated with 20 um LY294002 for 30 min prior to treatment with 100 ng/ml IGF-1 for 4 h.

Western blot

LNCaP cells (passage number 38) were serum-starved for 2 days. After serum starvation, cells were (A)left untreated, (B) treated with 100 ng/ml IGF-1 for 4h, or (C) incubated with 20 um LY294002 for 30 min prior to treatment with 100 ng/ml IGF-1 for 4 h.
LNCaP cells (passage number 38) were serum-starved for 2 days. After serum starvation, cells were (A)left untreated, (B) treated with 100 ng/ml IGF-1 for 4h, or (C) incubated with 20 um LY294002 for 30 min prior to treatment with 100 ng/ml IGF-1 for 4 h.

Requested From: United States
Date Requested: 12/11/2018
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