Human Apolipoprotein A-II

Goat

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

Highly purified apoLipoprotein Type A-II from human plasma.

Recognizes human apoLipoprotein A-II. Exhibits very low cross-reactivity with Type A-I, B, C-I, C-II, C-III, E and J apoLipoproteins or other human serum proteins.

• IHC
• IHC - Paraffin (5 µg/ml)
• Western blot (1:2500 - 1:5000)
• Immunoprecipitation
• ELISA (1:2500 - 1:5000)

Immunohistochemistry: LS-B2466 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2466 was determined to be 5 ug/ml.

0.125 M sodium borate, 0.075 M sodium chloride, 0.005 M EDTA, pH 8.0, 0.01% sodium azide, before the addition of glycerol to 40%. No stabilizing proteins added.

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P02652 NCBI: NM_001643 NP_001634.1