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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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APOE / Apolipoprotein E Antibody (aa305-317) IHC-plus™ LS-B2390

Note: This antibody replaces LS-C54521
Anti-APOE / Apolipoprotein E antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
APOE / Apolipoprotein E antibody (2µg/ml) staining of Human Brain (Hippocampus) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.1 ug/ml) staining of Human Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Anti-APOE / Apolipoprotein E antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
APOE / Apolipoprotein E antibody (2µg/ml) staining of Human Brain (Hippocampus) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.1 ug/ml) staining of Human Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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APOE / Apolipoprotein E Antibody (aa305-317) IHC-plus™ LS-B2390

Note: This antibody replaces LS-C54521
Antibody:
Goat Polyclonal to Human APOE / Apolipoprotein E
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B2390-50
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Human APOE / Apolipoprotein E
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Apolipoprotein E antibody LS-B2390 is an unconjugated goat polyclonal antibody to human Apolipoprotein E (APOE). Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.

Target
Human APOE / Apolipoprotein E
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (0.1 - 0.3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Peptide with sequence C-VGTSAAPVPSDNH, from the C Terminus of the protein sequence according to NP_000032.1.
Blocking Peptide
LS-E25851 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B2390. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa305-317
Specificity
Human APOE / Apolipoprotein E.
Usage
Immunohistochemistry: LS-B2390 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2390 was determined to be 2.5 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About APOE / Apolipoprotein E
P02649 NM_000041 NP_000032.1

Validation

Anti-APOE / Apolipoprotein E antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC Validated
Anti-APOE / Apolipoprotein E antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-APOE / Apolipoprotein E antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

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Requested From: United States
Date Requested: 6/26/2019
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