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Catalog Number Size Price
LS-C355403-1 1 mg (5 mg/ml) $913 
APOB / Apolipoprotein B Antibody - Immunofluorescence analysis of Apolipoprotein B was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Apolipoprotein B (F2C9) Mouse Monoclonal Antibody at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

Monoclonal Mouse anti‑Human APOB / Apolipoprotein B Antibody (clone F2C9, IF) LS‑C355403

Monoclonal Mouse anti‑Human APOB / Apolipoprotein B Antibody (clone F2C9, IF) LS‑C355403

Antibody:
APOB / Apolipoprotein B Mouse anti-Human Monoclonal (F2C9) Antibody
Application:
IF, IP, ELISA, RIA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$913
LS-C355403-1
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
APOB / Apolipoprotein B Mouse anti-Human Monoclonal (F2C9) Antibody
Application:
IF, IP, ELISA, RIA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Apolipoprotein B antibody LS-C355403 is an unconjugated mouse monoclonal antibody to human Apolipoprotein B (APOB). Validated for ELISA, IF, IP and RIA.
Target
Human APOB / Apolipoprotein B
Synonyms
APOB | ApoB-100 | Apolipoprotein B-48 | ApoB-48 | Apolipoprotein B | FLDB | LDLCQ4 | Mutant Apo B 100 | Apo B-100 | Apolipoprotein B-100 | Apolipoprotein B48
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
F2C9
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Purified human serum LDL.
Specificity
The antibody targets Apolipoprotein B in ELISA, IP and RIA applications and shows reactivity with Human samples. It detects Apolipoprotein B which has a predicted molecular weight of approximately 502 kD.
Applications
  • Immunofluorescence
  • Immunoprecipitation
  • ELISA
  • Radioimmunoassay
Presentation
PBS, pH 7.4, 0.09% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About APOB / Apolipoprotein B
P04114 NM_000384 NP_000375.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/18/2024