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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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APLNR/ Apelin Receptor / APJ Antibody (Cytoplasmic Domain) IHC‑plus™ LS‑A63
APJ antibody LS-A63 is an unconjugated rabbit polyclonal antibody to APJ (APLNR / Apelin Receptor) from human, hamster and horse. Validated for IHC. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-A63-50
50 µg (1 mg/ml)
$425
In Stock for Immediate Shipment

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Product Description

APJ antibody LS-A63 is an unconjugated rabbit polyclonal antibody to APJ (APLNR / Apelin Receptor) from human, hamster and horse. Validated for IHC. Tested on 20 paraffin-embedded human tissues.

Specifications

Target
Human APLNR/ Apelin Receptor / APJ
Synonyms
APLNR, AGTRL1, Angiotensin receptor-like 1, Angiotensin II receptor-like 1, Apelin receptor, APJ, APJ (apelin) receptor, APJR, G protein-coupled receptor APJ, HG11 orphan receptor, HG11, APJ receptor, G-protein coupled receptor APJ
Host
Rabbit
Reactivity
Human, Hamster, Horse (tested or 100% immunogen sequence identity)
Predicted
Monkey, Mouse, Rat, Bat, Rabbit (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (4 - 7 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Failed Applications
  • Western blot
Immunogen
Synthetic 16 amino acid peptide from 1st cytoplasmic domain of human Apelin Receptor. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Hamster, Elephant, Panda, Horse (100%); Orangutan, Marmoset, Mouse, Rat, Bat, Rabbit (94%).
Blocking Peptide
LS-E29119 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A63. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
Cytoplasmic Domain
Specificity
Human Apelin Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About APLNR/ Apelin Receptor / APJ
Receptor for apelin coupled to G proteins that inhibit adenylate cyclase activity and plays a role in various processes in adults such as regulation of blood pressure, heart contractility, and heart failure. Also plays a key role in early development such as gastrulation and heart morphogenesis by acting as a receptor for APELA hormone. Alternative coreceptor with CD4 for HIV-1 infection; may be involved in the development of AIDS dementia. P35414 NM_005161 NP_005152.1


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Images

Immunohistochemistry

Anti-Apelin Receptor antibody LS-A63 IHC of human brain, astrocyte. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-Apelin Receptor antibody LS-A63 IHC of human brain, astrocyte. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-Apelin Receptor antibody LS-A63 IHC of human brain, astrocyte. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-Apelin Receptor antibody LS-A63 IHC of human brain, astrocyte. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-Apelin Receptor antibody LS-A63 IHC of human brain, astrocyte. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-Apelin Receptor antibody LS-A63 IHC of human brain, astrocyte. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-Apelin Receptor antibody LS-A63 IHC of human brain, astrocyte. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-Apelin Receptor antibody LS-A63 IHC of human brain, astrocyte. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Requested From: United States
Date Requested: 10/17/2018

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