APH-1 antibody LS-C3769 is an unconjugated rabbit polyclonal antibody to APH-1 (APH1A) from human and mouse. Validated for IP and WB.
Human APH1A / APH-1
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
- Western blot (1 - 3 µg/ml)
- (applications tested for the base form of this product only)
APH1A / APH-1 antibody was raised against synthetic peptide derived from the C-terminal region of human Aph-1a.
Recognizes human Aph-1a at ~29kD. A fragment of Aph-1a may also be observed at ~23kD. Recognizes only the long isoform of Aph-1a. Reactivity has been confirmed with human Aph-1a- transfected N2a mouse neuroblastoma lysates. Bands representing both endogenous and exogenous Aph-1a were observed. The non-transfected N2a cell lysates only showed endogenous Aph-1a expression. Species cross-reactivity: human and mouse.
Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 1-3 ug/ml. Immunoprecipitation: 7-10 ug/IP reaction.
PBS, pH 7.4, 0.1% Sodium Azide, 40% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.