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Catalog Number Size Price
LS-C777283-100 100 µg (1 mg/ml) $479 
APG5 / ATG5 Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG5 Polyclonal Antibody. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG5 Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 40X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG5 Antibody (D) Composite.
APG5 / ATG5 Antibody - Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~32.4kDa ATG5 protein using Rabbit Anti-ATG5 Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG5 Polyclonal Antibody  at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~32.4kDa. Other Band(s): ~105kDa.
APG5 / ATG5 Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG5 Polyclonal Antibody. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG5 Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 40X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG5 Antibody (D) Composite.
APG5 / ATG5 Antibody - Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~32.4kDa ATG5 protein using Rabbit Anti-ATG5 Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG5 Polyclonal Antibody  at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~32.4kDa. Other Band(s): ~105kDa.
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Polyclonal Rabbit anti‑Human APG5 / ATG5 Antibody (Atto 488, IF, WB) LS‑C777283

Polyclonal Rabbit anti‑Human APG5 / ATG5 Antibody (Atto 488, IF, WB) LS‑C777283

Antibody:
APG5 / ATG5 Rabbit anti-Human Polyclonal (Atto 488) Antibody
Application:
IF, WB
Reactivity:
Human
Format:
Atto 488, Unmodified
Other formats:
Price
Catalog Number
$479
LS-C777283-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
APG5 / ATG5 Rabbit anti-Human Polyclonal (Atto 488) Antibody
Application:
IF, WB
Reactivity:
Human
Format:
Atto 488, Unmodified
Other formats:

Specifications

Description
ATG5 antibody LS-C777283 is an Atto 488-conjugated rabbit polyclonal antibody to human ATG5 (APG5). Validated for IF and WB.
Target
Human APG5 / ATG5
Synonyms
ATG5 | APG5 | APG5-LIKE | APG5L | Apoptosis specific protein | Apoptosis-specific protein | Autophagy related 5 | HAPG5 | ASP | Autophagy protein 5
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Atto 488. Also available conjugated with APC, Atto 390, Atto 594, Biotin, FITC, PerCP, RPE.
Purification
Peptide Affinity Purified
Modifications
Unmodified
Immunogen
Synthetic peptide from the N-Terminus of human ATG5
Specificity
Predicted molecular weight at ~32.4 kDa. Observed molecular weights at ~48-60 kDa based on ATG12-ATG5 heterodimer.
Applications
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
  • Applications tested for the base form of this product only
Usage
Applications should be user optimized.
Presentation
PBS, 0.09% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About APG5 / ATG5
Q9H1Y0 NM_004849 NP_004840.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/18/2024