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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C156569-400 400 µl $325 
APG12 / ATG12 Antibody - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
APG12 / ATG12 Antibody - Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with Autophagy APG12L antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
APG12 / ATG12 Antibody - Fluorescent image of U251 cells stained with ATG12 antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated ATG12 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG12 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
APG12 / ATG12 Antibody - Western blot of anti-APG12L antibody in HeLa cell lysate. APG12L (arrow) was detected using the purified antibody.
APG12 / ATG12 Antibody - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
APG12 / ATG12 Antibody - Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with Autophagy APG12L antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
APG12 / ATG12 Antibody - Fluorescent image of U251 cells stained with ATG12 antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated ATG12 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG12 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
APG12 / ATG12 Antibody - Western blot of anti-APG12L antibody in HeLa cell lysate. APG12L (arrow) was detected using the purified antibody.
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Polyclonal APG12 / ATG12 Antibody (aa1‑30, IHC, IF, WB) LS‑C156569

Polyclonal APG12 / ATG12 Antibody (aa1‑30, IHC, IF, WB) LS‑C156569

Antibody:
Rabbit Polyclonal to Human APG12 / ATG12
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$325 
LS-C156569-400
400 µl
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human APG12 / ATG12
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
ATG12 antibody LS-C156569 is an unconjugated rabbit polyclonal antibody to human ATG12 (APG12 ). Validated for IF, IHC and WB. Cited in 1 publication.
Target
Human APG12 / ATG12
Synonyms
ATG12 | Autophagy-related protein 12 | APG12L | Autophagy related 12 | FBR93 | APG12 | APG12-like | Ubiquitin-like protein ATG12 | HAPG12
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:200)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E5641 - Lyophilized - 500 µg - $360.00
Immunizing peptide used to generate LS-C156569. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa1-30
Specificity
This ATG12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human ATG12.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About APG12 / ATG12
O94817 NM_004707 NP_004698.3

Publications (1)

Activation of autophagy in mesenchymal stem cells provides tumor stromal support. Sanchez CG, Penfornis P, Oskowitz AZ, Boonjindasup AG, Cai DZ, Dhule SS, Rowan BG, Kelekar A, Krause DS, Pochampally RR. Carcinogenesis. 2011 32:964-72.

Customer Reviews (0)


Requested From: United States
Date Requested: 2/23/2020