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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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APC Antibody (aa1‑433) LS‑C3765
APC antibody LS-C3765 is an unconjugated mouse monoclonal antibody to human APC. Validated for IHC and WB.
Antibody
Catalog
Size
Price
LS-C3765-50
50 µg (0.55 mg/ml)
$455

Popular APC Products

Anti-APC antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2788 concentration 10 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Western blot analysis of APC (pS2054) expression in HeLa (A); Caco2 (B); SW480 (C); MCF7 (D); SP2/0 (E); PC12 (F) whole cell lysates.
Species: Human, Monkey, Mouse, Rat, Pig
Applications: Western blot
Western blot of APC antibody
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot, ELISA
Detection of Human APC by Immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-APC used at a dilution of 1:500.
Species: Human, Mouse, Rat, Bovine, Dog, Chicken, Xenopus, Zebrafish
Applications: IHC, IHC - Paraffin, Immunofluorescence
Anti-APC antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7592 dilution 1:100.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay

Product Description

APC Antibody (aa1-433) for IHC, WB/Western LS-C3765

Specifications

Target
Human APC
Synonyms
APC, Adenomatosis polyposis coli, Adenomatous polyposis coli, BTPS2, Deleted in polyposis 2.5, DP2, DP2.5, FPC, GS, Protein APC, PPP1R46, DP3
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Frozen
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
APC antibody was raised against synthetic peptide corresponding to aa1-433 N-terminal fragment of human APC (Adenomatous Polyposis Coli gene Chr 5q) (coupled to maltose binding protein). Cellular localization: cytoplasmic and nuclear.
Epitope
aa1-433
Specificity
Recognizes the N-terminal region of the human APC protein. Epitope Mapping: Performed by differential in vitro expression of the N-terminal region of the APC gene by using the protein truncation test. Found to bind to APC in the region between aa135-422 (exon 2-3). Species cross-reactivity: Does not cross-react with mouse.
Usage
Suitable for use in Immunohistochemistry and Western Blot. Immunohistochemistry: Acetone fixed, frozen sections. Positive control: Colon cell line HCT116, SW480 and SW837 extracts.
Presentation
PBS, pH 7.2, 40% Glycerol
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About APC
Tumor suppressor. Promotes rapid degradation of CTNNB1 and participates in Wnt signaling as a negative regulator. APC activity is correlated with its phosphorylation state. Activates the GEF activity of SPATA13 and ARHGEF4. Plays a role in hepatocyte growth factor (HGF)-induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. P25054 NM_000038 NP_000029.2


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Requested From: United States
Date Requested: 10/15/2018

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