APC antibody LS-C3765 is an unconjugated mouse monoclonal antibody to human APC. Validated for IHC and WB.
(tested or 100% immunogen sequence identity)
Protein A purified
- IHC - Frozen
- Western blot
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APC antibody was raised against synthetic peptide corresponding to aa1-433 N-terminal fragment of human APC (Adenomatous Polyposis Coli gene Chr 5q) (coupled to maltose binding protein). Cellular localization: cytoplasmic and nuclear.
Recognizes the N-terminal region of the human APC protein. Epitope Mapping: Performed by differential in vitro expression of the N-terminal region of the APC gene by using the protein truncation test. Found to bind to APC in the region between aa135-422 (exon 2-3). Species cross-reactivity: Does not cross-react with mouse.
Suitable for use in Immunohistochemistry and Western Blot. Immunohistochemistry: Acetone fixed, frozen sections. Positive control: Colon cell line HCT116, SW480 and SW837 extracts.
PBS, pH 7.2, 40% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.