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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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APBB1 / FE65 Antibody (aa1‑708) LS‑C126761
FE65 antibody LS-C126761 is an unconjugated mouse polyclonal antibody to human FE65 (APBB1). Validated for IF and WB.
Antibody
Catalog
Size
Price
LS-C126761-50
50 µg
$560

Popular APBB1 / FE65 Products

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Immunohistochemistry - Paraffin Image
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Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human
Applications: Immunofluorescence, Western blot, ELISA
Immunofluorescence analysis of Fe65 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Fe65 Rabbit Polyclonal Antibody at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
Species: Mouse
Applications: Immunofluorescence, Western blot

Product Description

FE65 antibody LS-C126761 is an unconjugated mouse polyclonal antibody to human FE65 (APBB1). Validated for IF and WB.

Specifications

Target
Human APBB1 / FE65
Synonyms
APBB1, FE65, Adaptor protein FE65a2, Protein Fe65, Stat-like protein, MGC:9072, RIR
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Applications
  • Immunofluorescence
  • Western blot
Immunogen
APBB1 / FE65 antibody was raised against aPBB1 (NP_663722.1, 1-708aa) full-length human protein.
Epitope
aa1-708
Specificity
Recognizes human APBB1.
Usage
Suitable for use in Western Blot and Immunofluorescence.
Presentation
PBS, pH 7.2
Storage
May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for up to 1 year.
Restrictions
For research use only.
About APBB1 / FE65
Transcription coregulator that can have both coactivator and corepressor functions. Adapter protein that forms a transcriptionally active complex with the gamma-secretase-derived amyloid precursor protein (APP) intracellular domain. Plays a central role in the response to DNA damage by translocating to the nucleus and inducing apoptosis. O00213 NM_001164 NP_001155.1


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Requested From: United States
Date Requested: 11/15/2018

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