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Catalog Number Size Price
LS-C750630-20 20 µl $287 
LS-C750630-50 50 µl $341 
LS-C750630-100 100 µl $431 
LS-C750630-200 200 µl $539 
AP50 / AP2M1 Antibody - Western blot analysis of extracts of 293T and NIH/3T3 cells, using Phospho-AP2M1-T156 antibody at 1:1000 dilution. 293T cells were treated by Calyculin A for 30 minutes after serum-starvation overnight.NIH/3T3 cells were treated by Hydrogen Peroxide (2nM) for 15 minutes after serum-starvation overnight. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. Blocking buffer: 3% BSA.An ECL Kit was used for detection and the exposure time was 30s.

Polyclonal Rabbit anti‑Human AP50 / AP2M1 Antibody (phospho‑Thr156, WB) LS‑C750630

Polyclonal Rabbit anti‑Human AP50 / AP2M1 Antibody (phospho‑Thr156, WB) LS‑C750630

Antibody:
AP50 / AP2M1 Rabbit anti-Human Polyclonal (pThr156) Antibody
Application:
WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$287
LS-C750630-20
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
AP50 / AP2M1 Rabbit anti-Human Polyclonal (pThr156) Antibody
Application:
WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
AP2M1 antibody LS-C750630 is an unconjugated rabbit polyclonal antibody to AP2M1 (AP50) (pThr156) from human. It is reactive with human, mouse and rat. Validated for WB.
Target
Human AP50 / AP2M1
Synonyms
AP2M1 | Adaptin-mu2 | AP-2 complex subunit mu | AP-2 mu 2 chain | AP-2 mu chain | AP50 | CLAPM1 | Mu2 | KIAA0109 | HA2 50 kDa subunit
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Epitope
pThr156
Applications
  • Western blot (1:500 - 1:2000)
Usage
The predicted MW is 49kDa, while the observed MW by Western blot was 50kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About AP50 / AP2M1
Q96CW1 NM_004068 NP_004059.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/16/2024