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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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ANXA4 / Annexin IV Antibody (aa108‑157) IHC‑plus™ LS‑B11479
Note: This antibody replaces LS-C101638
Annexin IV antibody LS-B11479 is an unconjugated rabbit polyclonal antibody to Annexin IV (ANXA4) from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B11479-50
50 µg
$485

Popular ANXA4 / Annexin IV Products

Anti-Annexin IV antibody IHC of human liver, bile duct. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4279 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Immunohistochemistry of Human gastric cancer using ANXA4 Polyclonal Antibody at dilution of 1:25.
Species: Human, Mouse, Rat
Applications: IHC, Western blot, ELISA
Anti-ANXA4 / Annexin IV antibody IHC staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11479 concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Chimpanzee, Gibbon, Mouse, Rat, Bat, Bovine, Dog, Pig
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species:
Applications: IHC, Immunofluorescence, Western blot, ELISA
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot

Product Description

ANXA4 / Annexin IV Antibody (aa108-157) for IHC, WB/Western IHC-plus™ LS-B11479

Specifications

Target
Human ANXA4 / Annexin IV
Synonyms
ANXA4, 35-beta calcimedin, ANX4, Annexin A4, Annexin IV, Annexin-4, Chromobindin-4, Endonexin I, Lipocortin IV, p32.5, PIG28, Proliferation-inducing gene 28, Protein II, ZAP36, p33/41, PAP-II, PP4-X
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bat, Bovine, Dog, Pig (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with FITC, Biotin, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide located between aa108-157 of human ANXA4 (P09525, NP_001144). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Dog, Bovine, Bat, Pig (100%); Marmoset, Elephant, Rabbit, Horse, Guinea pig (92%); Mouse, Rat, Zebra finch (85%); Xenopus, Frog (84%).
Blocking Peptide
LS-E13101 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B11479. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa108-157
Specificity
Human ANXA4 / Annexin IV
Usage
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About ANXA4 / Annexin IV
Annexin IV (ANX4) belongs to the annexin family of calcium-dependent phospholipid binding proteins. Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. ANX4 has 45 to 59% identity with other members of its family and shares a similar size and exon-intron organization. P09525 NM_001153 NP_001144.1


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Images

Immunohistochemistry

Anti-ANXA4 / Annexin IV antibody IHC staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11479 concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-ANXA4 / Annexin IV antibody IHC staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11479 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

ANXA4 / Annexin IV antibody western blot of Fetal Lung lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ANXA4 / Annexin IV antibody western blot of Fetal Lung lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-ANXA4 / Annexin IV antibody IHC staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11479 concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-ANXA4 / Annexin IV antibody IHC staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11479 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

ANXA4 / Annexin IV antibody western blot of Fetal Lung lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ANXA4 / Annexin IV antibody western blot of Fetal Lung lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-ANXA4 / Annexin IV antibody IHC staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11479 concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-ANXA4 / Annexin IV antibody IHC staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11479 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

ANXA4 / Annexin IV antibody western blot of Fetal Lung lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ANXA4 / Annexin IV antibody western blot of Fetal Lung lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-ANXA4 / Annexin IV antibody IHC staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11479 concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-ANXA4 / Annexin IV antibody IHC staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11479 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

ANXA4 / Annexin IV antibody western blot of Fetal Lung lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ANXA4 / Annexin IV antibody western blot of Fetal Lung lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 10/18/2018

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