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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C596-100 100 µg (0.5 mg/ml) $335 
ANTXR1 / TEM8 Antibody - Western blot detection of TEM8 in TEM8 transfected cell lysate using antibody at 1 ug/ml.

Monoclonal ANTXR1 / TEM8 Antibody (clone 200C1339(SB20), aa481‑497, WB) LS‑C596

Monoclonal ANTXR1 / TEM8 Antibody (clone 200C1339(SB20), aa481‑497, WB) LS‑C596

Note: This antibody replaces LS-C92696, LS-C18057
Antibody:
Mouse Monoclonal to Human ANTXR1 / TEM8
Application:
WB
Reactivity:
Human, Monkey, Dog, Rabbit
Format:
Unconjugated, Unmodified
Price
Catalog Number
$335 
LS-C596-100
100 µg
0.5 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Mouse Monoclonal to Human ANTXR1 / TEM8
Application:
WB
Reactivity:
Human, Monkey, Dog, Rabbit
Format:
Unconjugated, Unmodified

Specifications

Description
TEM8 antibody LS-C596 is an unconjugated mouse monoclonal antibody to TEM8 (ANTXR1) (aa481-497) from human, dog, monkey and other species. Validated for WB.
Target
Human ANTXR1 / TEM8
Synonyms
ANTXR1 | Tumor endothelial marker 8 | Anthrax toxin receptor 1 | TEM8
Host
Mouse
Reactivity
Human, Monkey, Dog, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Bat, Pig (at least 90% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
200C1339(SB20)
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • Western blot (1 - 3 µg/ml)
Immunogen
This monoclonal antibody was developed by immunizing mice with a synthetic peptide containing amino acids 481-497 (CINFTRVKNNQPAKYPL) of human TEM8. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Dog, Panda, Rabbit (100%); Mouse, Rat, Bat, Elephant, Pig (94%); Horse, Opossum (88%).
Epitope
aa481-497
Specificity
TEM8 is observed at ~80 kD in western blots of HUVEC cells (Hotchkiss et al, 2005) and as an 80 kD and 85 kD double in human colon cell lysates (Nanada et al, 2004). The size difference is thought to be due to glycosylation as a 70 kD band was seen when cell extracts in Nanda et al (2004) were treated with a glycosidase cocktail. Nanda et al (2004) note a passage dependent loss of TEM8 in HUVEC suggesting that that TEM8 may be a specific marker of the endothelial cell (EC) phenotype which is lost as primary EC's age in culture and de-differentiate.
Presentation
PBS, 0.05% Sodium Azide, 0.2% Gelatin
Storage
Short term: 4°C; Long term: -70°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ANTXR1 / TEM8
Q9H6X2 NM_018153 NP_060623.2

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Requested From: United States
Date Requested: 3/29/2020